Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Economic Debate On Spatial Functional Linkages And Its Application to Key Spatial Development Challanges In Poland [PDF]
This article presents a synthesis of today's world economic knowledge and the results of the analyses concerning: a) the nature of spatial functional linkages, including network structures, b) diversity of approaches concerning the application of spatial
Jacek Zaucha
core
Regional-level spatial accessibility and diabetes management in South Korea: a multilevel analysis using national longitudinal data. [PDF]
Ha R, Kim CY, Jung-Choi K.
europepmc +1 more source
Examining the impact of spatial accessibility to rehabilitation facilities on the degree of disability: A heterogeneity perspective. [PDF]
Qiu N, Zhang T, Cheng J.
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Measuring spatial accessibility for wheelchair users: A case study in a Chinese campus. [PDF]
Huang Y +5 more
europepmc +1 more source
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin +12 more
wiley +1 more source
Methodology for the Assessment of Spatial Economic Impacts of Transport Projects and Policies [PDF]
In this study we develop an integrated econometric and CGE modelling framework for transport projects and transport policies at the European level by integrating network, regional economic and macro-economic impacts.
Johannes Brocker, d’Artis Kancs
core
Impact of spatial accessibility to primary care physicians on health care outcomes and costs. [PDF]
Weng YX, Hsieh CC, Liao HC, Tung YC.
europepmc +1 more source

