Results 111 to 120 of about 1,677,553 (308)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

From buildings to cities: techniques for the multi-scale analysis of urban form and function

open access: yes, 2010
The built environment is a significant factor in many urban processes, yet direct measures of built form are seldom used in geographical studies. Representation and analysis of urban form and function could provide new insights and improve the evidence
Smith, D., Crooks, A.
core  

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Reconstructing Historical Landsat Time Series Using a Transformer-Based Deep Learning Approach: A Case Study in the Canadian Prairies Region

open access: yesCanadian Journal of Remote Sensing
The Landsat program is crucial for monitoring environmental changes, benefiting from its long record of moderate-spatial-resolution imagery, well-established calibration, and commitment to providing open-access data.
Masoud Babadi Ataabadi   +3 more
doaj   +1 more source

The use of multi-criteria evaluation in spatial policy

open access: yes, 2007
Conventional multi-criteria evaluation methods have largely been non-spatial. They use average or total impacts that are considered appropriate for the entire area under consideration.
Van Herwijnen, Marjan   +2 more
core  

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

open access: yesFEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada   +5 more
wiley   +1 more source

Applying Machine Learning Algorithms for Spatial Modeling of Flood Susceptibility Prediction over São Paulo Sub-Region

open access: yesLand
Floods are among the most destructive natural hazards globally, necessitating the identification of flood-prone areas for effective disaster risk management and sustainable urban development.
Temitope Seun Oluwadare   +5 more
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Spatial Point Pattern Analysis and Industry Concentration [PDF]

open access: yes
Traditional measures of spatial industry concentration are restricted to given areal units. They do not make allowance for the fact that concentration may be differently pronounced at various geographical levels. Methods of spatial point pattern analysis
Hans-Friedrich Eckey   +2 more
core  

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