Results 101 to 110 of about 326,953 (255)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Spherical Acoustic Spatial Entropy: Predicting Acoustic Scene Complexity in Virtual Environments
IEEE Open Journal of Signal ProcessingThe objective quantification of Acoustic Scene Complexity (ASC) remains a significant challenge. While existing entropy-based metrics capture spectro-temporal variability, a metric accounting for the spatial distribution of sources has been lacking.
Luca Resti +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa +2 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Optimization of VAWT installation with spatial and temporal complexity considerations
Frontiers in Energy ResearchThe constant need for fuel to meet the commercial sector’s ever-increasing demand has driven researchers to discover and optimize renewable energy resources, paving the way for sustainable production of reliable and clean energy resources.
Mohammed Alqahtani
doaj +1 more source
FEBS Open Bio, EarlyView.ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
We provide a new geometric characterization for the spine of untwisted outer space of a right-angled Artin group, constructed by Charney, Stambaugh, and Vogtmann. We realize the spine as the natural simplicial complex associated with the category of spatial cube complexes, a new class of locally CAT(0) cube complexes with a simple definition in terms ...
openaire +2 more sources
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Substrate Spatial Complexity Analysis for the Prediction of Ventricular Arrhythmias in Patients With Ischemic Cardiomyopathy. [PDF]
Circ Arrhythm Electrophysiol, 2020 Okada DR +7 more
europepmc +1 more source