Results 61 to 70 of about 2,481,877 (286)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Spatial data interpolation

open access: yes, 2022
Massive data collection has been carried out in both information gathering and decision making in real-time. Due to the nature of the data type (spatial or spatio-temporal), treatment and interpolation become essential steps in this process. In this context, computer systems capable of generating thematic maps are used, through the interpolation of the
Afonso Paiva   +9 more
openaire   +1 more source

Comparison of interpolation methods based on Geographic Information System (GIS) in the spatial distribution of seawater intrusion

open access: yesJurnal Natural, 2020
The study of monitoring seawater intrusion and groundwater quality in a coastal area needs to be done regularly to prevent the clean water crisis problems in the future.
MAULINA TANJUNG   +2 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

open access: yesFEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose   +9 more
wiley   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

open access: yesFEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss   +7 more
wiley   +1 more source

Spatio-Temporal Median Polish Kriging with ARIMA Integration for Monthly Precipitation Interpolation in East Kalimantan

open access: yesJTAM (Jurnal Teori dan Aplikasi Matematika)
Precipitation can lead to disasters like droughts and floods, necessitating accurate interpolation methods. Traditional spatio-temporal kriging often struggles with outliers, which can reduce estimation reliability.
Friendtika Miftaqul Jannah   +2 more
doaj   +1 more source

Comparison of Inverse Distance Weighted and Natural Neighbor Interpolation Method at Air Temperature Data in Malang Region

open access: yesCauchy: Jurnal Matematika Murni dan Aplikasi, 2018
The purpose of this study was to compare the results of Inverse Distance Weighted (IDW) and Natural Neighbor interpolation methods for spatial data of air temperature in the Malang Region.
Jaka Pratama Musashi   +2 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Influence of different data densities on the mapping of agroclimatological parameters - case of precipitation

open access: yesActa Agriculturae Slovenica, 1998
Different spatial densities of precipitation data were used to illustrate the influence of data density on spatial information about this parameter. Four maps of long term precipitation data (1961-1990) for Slovenia were produced: on a base of all, half,
Klemen BERGANT, Lučka KAJFEŽ-BOGATAJ
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

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