Results 141 to 150 of about 365,427 (304)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Spatial mode discrimination using second harmonic generation

, 2007
Second harmonic generation can be used as a technique for controlling the spatial mode structure of optical beams. We demonstrate experimentally the generation of higher order spatial modes, and that it is possible to use nonlinear phase matching as a ...
Bachor, Hans-A., Lassen, Mikael Østergaard, harb, Charles, Pulford, David, Delaubert, Vincent   +4 more
core   +1 more source

Residue feature analysis with empirical mode decomposition for mining spatial sequential patterns from serial remote sensing images

Научно-технический вестник информационных технологий, механики и оптики
An extensive growth of serial remote sensing images paves the way for abundant data intended for the sequential spatial pattern determination in several fields like monitoring of agriculture, development of urban areas, and the vegetative area.
R. A. Preethi, G. Anandharaj
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Spectral properties and modes of surface microcavities

, 2009
This thesis describes the experimental investigations into the transverse mode structure of nearly hemispherical microcavities. The nearly hemispherical microcavity structures are fabricated electrocemically through a template of self assembled latex ...
D'Alessandro, G., Baumberg, J.J., Kaczmarek, M., Pennington, R.C.   +3 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Full mode and attribution mode in environmental analysis

, 2000
Several tools exist for the analysis of the environmental impacts of chains or networks of processes. These relatively simple tools include materials flow accounting (MFA), substance flow analysis (SFA), life-cycle assessment (LCA), energy analysis, and ...
De Udo Haes, Helias A., Heijungs, Reinout; id_orcid, Heijungs, Reinout, Udo de Haes, H.A., Huppes, G., Heijungs, R., Voet, E. van der, Van Der Voet, Ester, Hettelingh, Jean Paul, Huppes, Gjalt, Hettelingh, J.-P.   +10 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Methodology for the Assessment of Spatial Economic Impacts of Transport Projects and Policies [PDF]

In this study we develop an integrated econometric and CGE modelling framework for transport projects and transport policies at the European level by integrating network, regional economic and macro-economic impacts.
Johannes Brocker, d’Artis Kancs
core