Results 141 to 150 of about 160,073 (300)
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Effectiveness of conditional punishment for the evolution of public cooperation
, 2013 Collective actions, from city marathons to labor strikes, are often mass-driven and subject to the snowball effect. Motivated by this, we study evolutionary advantages of conditional punishment in the spatial public goods game.
Perz, Matjaž, Szolnoki, Attila
core +1 more source
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Not all sprawl - Evolution of employment centers in Los Angeles, 1980 - 2000 [PDF]
Are contemporary metropolitan regions becoming more dispersed? The collective set of urban models tells us two stories. The first is that changes in the structure of the economy, ever faster and cheaper information and communications technologies, and ...
Genevieve Giuliano, Christian Redfearn
core
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
Long Term Evolution of the Size Distribution of Portuguese Cities [PDF]
In this paper we study the evolution of the Portuguese urban system from 1864 to 2001. We apply the rank-size model and use rank-size estimates to describe the evolution of city-size hierarchy.
Ana Paula Delgado, Isabel Maria Godinho
core
, 2015 This study was made possible through the kind financial support of the São Paulo Research Foundation - http://fapesp.br/en (Fundação de Amparo à Pesquisa do Estado de São Paulo - Fapesp 2013/15280-9).Seasonally dry tropical plant formations (SDTF) are ...
Marcelo Freire Moro +23 more
core +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
, 2005 Many dissipative optical systems support patterns. Dissipative solitons are generally found where a pattern coexists with a stable unpatterned state. We consider such phenomena in driven optical cavities containing a nonlinear medium (cavity solitons ...
Firth, William, Ackemann, Thorsten
core
FEBS Open Bio, EarlyView.We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa +2 more
wiley +1 more source