Generation of Polyclonal Specific Antibodies
Generation of antibodies specific for a protein of interest is a common method in many disciplines. This protocol details the steps in production of a polyclonal antibody in rabbits using a bacterially expressed fusion protein as an antigen. The protocol
Maureen Wirschell, Mary Porter
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Features of humoral answer in experimental animal tularemia with different sensitivity to infection
Tularemia is an anthropozoonotic infection caused by Francisella tularensis. In clinical and sanitary-epidemiological practice, traditional diagnostics methods in tularemia are based on serological assays for detecting specific antibodies, allowing to ...
A. A. Gorbatov +10 more
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Specific antibodies against binase: Preparation and application
Secreted ribonuclease of Bacillus pumilus (binase) is a promising antitumor agent with established selective cytotoxicity against various types of cancer cells expressing specific oncogenes.
V.I. Vershinina +4 more
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Monoclonal Antibodies against the Drosophila Nervous System [PDF]
A panel of 148 monoclonal antibodies directed against Drosophila neural antigens has been prepared by using mice immunized with homogenates of Drosophila tissue. Antibodies were screened immunohistochemically on cryostat sections of fly heads.
Benzer, Seymour +4 more
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COMPARISON OF THE PRIMARY AND SECONDARY HUMORAL IMMUNE RESPONSE TO VACCINATION BY “PRIORIX”
. Twenty healthy children were vaccinated, and then in a 5 years they were revaccinated with Priorix (measles, mumps, rubella vaccine), according to the national immunization calendar.
A. P. Toptygina, V. A. Alioshkin
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Detection of antibody-dependent complement mediated inactivation of both autologous and heterologous virus in primary HIV-1 infection [PDF]
Specific CD8 T-cell responses to human immunodeficiency virus type 1 (HIV-1) are induced in primary infection and make an important contribution to the control of early viral replication.
Aasa-Chapman, M.M.I. +10 more
core
Development of Vasoinhibin-Specific Monoclonal Antibodies
Vasoinhibin is a protein hormone with antiangiogenic, antivasodilatatory, and antivasopermeability effects generated by the proteolytic cleavage of prolactin.
Nils Müller +8 more
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Unraveling the Role of Allo-Antibodies and Transplant Injury. [PDF]
Alloimmunity driving rejection in the context of solid organ transplantation can be grossly divided into mechanisms predominantly driven by either T cell-mediated rejection (TCMR) and antibody-mediated rejection (ABMR), though the co-existence of both ...
Matsuda, Yoshiko, Sarwal, Minnie M
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Enzyme-linked immunosorbent assay for post-slaughter diagnosis of bovine leukosis
Postmortem diagnosis of enzootic bovine leukosis is made on the basis of the results of tests of biological materials from emergently slaughtered or fallen animals using pathomorphological, histological and molecular genetic methods that have some ...
A. R. Mustafayev, M. O. Baratov
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Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue. [PDF]
Two different monoclonal antibodies, characterized initially as binding synaptic terminal regions of rat brain, bind a 65,000-dalton protein, which is exposed on the outer surface of brain synaptic vesicles. Immunocytochemical experiments at the electron
Matthew, WD, Reichardt, LF, Tsavaler, L
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