Results 21 to 30 of about 1,894,326 (313)

Maximizing analytical precision: exploring the advantages of ratiometric strategy in fluorescence, Raman, electrochemical, and mass spectrometry detection

Frontiers in Analytical Science, 2023
Ratiometric strategy are an invaluable method that helps to detect and quantify analytes. This approach relies on measuring changes in the ratio of two or more signals to improve the accuracy and sensitivity of the results.
Manivannan Madhu, S. Santhoshkumar, Wei-Bin Tseng, Wei-Lung Tseng, Wei-Lung Tseng   +4 more
doaj   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Potential and application of X-ray fluorescence spectrometry to estimate iron and zinc concentration in potato tubers

Journal of Food Composition and Analysis, 2018
Breeding efforts to develop high iron and zinc potatoes require a fast and inexpensive technique to evaluate mineral concentrations in large numbers of samples.
P. Sosa, Georgia E. Guild, G. Burgos, M. Bonierbale, T. zum Felde   +4 more
semanticscholar   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

X-ray Fluorescence Analysis of Waste Sm-Co Magnets: A Rational Approach

Recycling, 2023
Determination of the chemical composition of waste Sm-Co magnets is required for their efficient recycling. The non-stereotypical composition of said magnets makes an analysis extremely challenging.
Alexandra Alexandrovna Arkhipenko, Galina Evgenievna Marina, Marina Sergeevna Doronina, Natalya Alexandrovna Korotkova, Vasilisa Borisovna Baranovskaya   +4 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Simple procedure for nutrient analysis of coffee plant with energy dispersive X-ray fluorescence spectrometry (EDXRF)

Scientia Agricola, 2013
Nutrient analysis is used to estimate nutrient content of crop plants to manage fertilizer application for sustained crop production. Direct solid analysis of agricultural and environmental samples by energy dispersive X-ray fluorescence spectrometry ...
Tiago Tezotto, José Laércio Favarin, Ana Paula Neto, Priscila Lupino Gratão, Ricardo Antunes Azevedo, Paulo Mazzafera   +5 more
doaj   +1 more source

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

Use of fluorescence imaging and spectrometry in detection of oral squamous cell carcinoma and oral potentially malignant disorders

Oral Oncology Reports
Objective: Changes in the autofluorescence of cancerous tissue in comparison to normal tissue due to alteration in the endogenous fluorophores and protein like NAD and FAD has been utilized in various oral cancer screening devices.
Vivek Nayyar, Pramila Thapa, Dalip Singh Mehta, Rahul Yadav, Krushna Bhatt, Varun Surya, Deepika Mishra   +6 more
doaj   +1 more source