Results 111 to 120 of about 1,818,239 (291)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Structural and functional glycosphingolipidomics by glycoblotting with aminooxy-functionalized gold nanoparticle [PDF]

, 2008
Glycosphingolipids (GSLs) synthesized in Golgi apparatus by sequential transfer of sugar residues to a ceramide lipid anchor are ubiquitously distributing on vertebrate plasma membranes.
Midori Abe, Noriko Nagahori, Shin-Ichiro Nishimura   +2 more
core   +1 more source

Toward precision mass measurements of neutron-rich nuclei relevant to $r$-process nucleosynthesis

, 2015
The open question of where, when, and how the heavy elements beyond iron enrich our Universe has triggered a new era in nuclear physics studies.\ Of all the relevant nuclear physics inputs, the mass of very neutron-rich nuclides is a key quantity for ...
Litvinov, Yu. A., Sun, B. H., Tanihata, I., Zhang, Y. H.   +3 more
core   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Rice origin traceability using mid-infrared and fluorescence spectral data fusion

Frontiers in Plant Science
This study overcomes the limitations of traditional single-spectroscopy techniques by constructing an intelligent discrimination system for rice geographic origin that integrates mid-infrared (MIR) and fluorescence (FLU) spectral feature fusion with ...
Changming Li, Changming Li, Yong Tan, Chunyu Liu, Xun Gao, Zhong Lv, Hongchen Zhang, Yong Zhang   +7 more
doaj   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Edible oils and fats authentication by Fourier transform Raman spectrometry. Analytical methods for the authentification of agro-food products. Gembloux (Belgium). 20 Oct 1999. [PDF]

Biotechnologie, Agronomie, Société et Environnement, 2000
The European project FAIR-CT96-5053 concerned the application of the Fourier transform Raman and infrared spectroscopy in food chemistry and quality control.
Baeten, V., Aparicio R.
doaj  

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Assessing a possibility of using atomic emission spectrometry for hypermucoid klebsiella differentiation

Инфекция и иммунитет
Various spectrometry (time-of-flight mass spectrometry, Raman spectrometry) types are used in the identification of microbial strains. Atomic emission spectrometry allows for the evaluation of the elemental sample composition, which can also be used in ...
Mariya G. Bikanova, Y. V. Zakharova, A. S. Sukhikh   +2 more
doaj   +1 more source