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Cryopreservation of Fish Sperm

2007
This chapter provides detailed descriptions of the protocol used for fish sperm cryopreservation. The principles described can be applied to any species, but details are also given for individual fish species. Successful cryopreservation of fish spermatozoa depends on a range of factors including the collection of high quality sperm, equilibration ...
Eugeny, Kopeika   +2 more
openaire   +2 more sources

Cryopreservation of epididymal stallion sperm

Cryobiology, 2014
Any event that makes semen collection or mating impossible, such as death, castration, or injury, may terminate a stallion's breeding career. Fortunately, stallion sperm which are capable of fertilization can be harvested from the epididymis, and frozen for future use.
M, Olaciregui   +5 more
openaire   +2 more sources

Concentrating human sperm before cryopreservation

Andrologia, 2009
To improve the quality of cryo-preserved sperm, ejaculated semen was concentrated prior to freezing by means of continuous-step density gradient centrifugation. Freezing was simplified by employing liquid nitrogen vapor with KS-II cryo-medium. The original semen (n = 32, 41 +/- 13 x 10(6)/ml, 17 +/- 11% motility) was improved by the processing to be 68
T, Kobayashi   +6 more
openaire   +2 more sources

Bacterial risk and sperm cryopreservation

Andrologia, 2004
Prior to sperm cryopreservation, French guidelines only recommend viral screening for serological status towards human immunodeficiency virus, hepatitis B and C viruses and Treponema palidum. The probability of semen infection by other bacterial pathogens is not taken into consideration by the current recommendations. The objective of the present study
R, Levy   +4 more
openaire   +2 more sources

Cryopreservation of microencapsulated canine sperm

Theriogenology, 2011
The objective was to develop a method for cryopreserving microencapsulated canine sperm. Pooled ejaculates from three beagle dogs were extended in egg yolk tris extender and encapsulated using alginate and poly-L-lysine at room temperature. The microcapsules were cooled at 4 °C, immersed in pre-cooled extender (equivalent in volume to the microcapsules)
Shambhu, Shah   +6 more
openaire   +2 more sources

Cryopreservation of Horse Sperm

Cryopreservation is currently the only strategy for long-term conservation of equine sperm. To get optimal post-thaw sperm survival, carefully following each step of the freezing protocol is crucial. First, one needs to obtain and exhaustively analyze an ejaculate of good sperm quality.
Jordi, Miró   +3 more
openaire   +2 more sources

Cryopreservation of sperm

2011
Abstract Malignant diseases in adolescence and younger adults such as testicular cancer, lymphomas and leukaemia have long-term survival rates of up to 80% if treated adequately. As a result, long-term quality of life, including reproductive health, has become increasingly important.
A. Kamischke, Eberhard Nieschlag
openaire   +1 more source

Cryopreservation of Plagiognathops microlepis sperm

Cryobiology, 2018
Sperm was collected from cultured male fish and cryopreserved in 0.25 ml straws for the study of sperm cryopreservation. Different parameters were evaluated, including extender, dilution ratio, cryoprotectant type and concentration, equilibrium time, cooling height (in a two-step cooling protocol), and thawing temperature.
Chen, Du   +3 more
openaire   +2 more sources

Sperm Cryopreservation in Boars

Cryopreservation is the procedure that allows preserving cells, including gametes, for long time periods while maintaining their functionality, through the use of cryogenic temperature. Currently, sperm cryopreservation is a crucial-assisted reproductive biotechnology in pigs since it is the only effective and efficient method for long-term ...
Inmaculada, Parrilla   +3 more
openaire   +2 more sources

Sperm Cryopreservation in Crustaceans

2020
Sperm cryopreservation facilities and reproductive management adds flexibility to breeding program design; it also allows mobilization over long distances, extends sperm half-life for several years, enables the use of selected males and prevent the reduction of genetic variability caused by inbreeding or environmental disasters.
Karina Morales-Ueno   +1 more
openaire   +1 more source

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