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Cryopreservation of Fish Sperm
2007This chapter provides detailed descriptions of the protocol used for fish sperm cryopreservation. The principles described can be applied to any species, but details are also given for individual fish species. Successful cryopreservation of fish spermatozoa depends on a range of factors including the collection of high quality sperm, equilibration ...
Eugeny, Kopeika +2 more
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Cryopreservation of epididymal stallion sperm
Cryobiology, 2014Any event that makes semen collection or mating impossible, such as death, castration, or injury, may terminate a stallion's breeding career. Fortunately, stallion sperm which are capable of fertilization can be harvested from the epididymis, and frozen for future use.
M, Olaciregui +5 more
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Concentrating human sperm before cryopreservation
Andrologia, 2009To improve the quality of cryo-preserved sperm, ejaculated semen was concentrated prior to freezing by means of continuous-step density gradient centrifugation. Freezing was simplified by employing liquid nitrogen vapor with KS-II cryo-medium. The original semen (n = 32, 41 +/- 13 x 10(6)/ml, 17 +/- 11% motility) was improved by the processing to be 68
T, Kobayashi +6 more
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Bacterial risk and sperm cryopreservation
Andrologia, 2004Prior to sperm cryopreservation, French guidelines only recommend viral screening for serological status towards human immunodeficiency virus, hepatitis B and C viruses and Treponema palidum. The probability of semen infection by other bacterial pathogens is not taken into consideration by the current recommendations. The objective of the present study
R, Levy +4 more
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Cryopreservation of microencapsulated canine sperm
Theriogenology, 2011The objective was to develop a method for cryopreserving microencapsulated canine sperm. Pooled ejaculates from three beagle dogs were extended in egg yolk tris extender and encapsulated using alginate and poly-L-lysine at room temperature. The microcapsules were cooled at 4 °C, immersed in pre-cooled extender (equivalent in volume to the microcapsules)
Shambhu, Shah +6 more
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Cryopreservation of Horse Sperm
Cryopreservation is currently the only strategy for long-term conservation of equine sperm. To get optimal post-thaw sperm survival, carefully following each step of the freezing protocol is crucial. First, one needs to obtain and exhaustively analyze an ejaculate of good sperm quality.Jordi, Miró +3 more
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2011
Abstract Malignant diseases in adolescence and younger adults such as testicular cancer, lymphomas and leukaemia have long-term survival rates of up to 80% if treated adequately. As a result, long-term quality of life, including reproductive health, has become increasingly important.
A. Kamischke, Eberhard Nieschlag
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Abstract Malignant diseases in adolescence and younger adults such as testicular cancer, lymphomas and leukaemia have long-term survival rates of up to 80% if treated adequately. As a result, long-term quality of life, including reproductive health, has become increasingly important.
A. Kamischke, Eberhard Nieschlag
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Cryopreservation of Plagiognathops microlepis sperm
Cryobiology, 2018Sperm was collected from cultured male fish and cryopreserved in 0.25 ml straws for the study of sperm cryopreservation. Different parameters were evaluated, including extender, dilution ratio, cryoprotectant type and concentration, equilibrium time, cooling height (in a two-step cooling protocol), and thawing temperature.
Chen, Du +3 more
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Sperm Cryopreservation in Boars
Cryopreservation is the procedure that allows preserving cells, including gametes, for long time periods while maintaining their functionality, through the use of cryogenic temperature. Currently, sperm cryopreservation is a crucial-assisted reproductive biotechnology in pigs since it is the only effective and efficient method for long-term ...Inmaculada, Parrilla +3 more
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Sperm Cryopreservation in Crustaceans
2020Sperm cryopreservation facilities and reproductive management adds flexibility to breeding program design; it also allows mobilization over long distances, extends sperm half-life for several years, enables the use of selected males and prevent the reduction of genetic variability caused by inbreeding or environmental disasters.
Karina Morales-Ueno +1 more
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