Results 111 to 120 of about 47,685 (161)
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Alkylating spin labels for spin-labelling of DNA
International Journal of Biochemistry, 1983Abstract 1. 1. Five alkylating spin labels, termed SL1, SL2, SL3, SL4 and SL5, have been synthesized and their suitability for spin-labelling of DNA was studied. 2. 2. It was found that one of them (SL5) lost its free radical moiety under the conditions of spin-labelling, thus giving DNA with no EPR signal. 3. 3.
I. Ivanov +4 more
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Photosynthesis Research, 2009
Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling.
Johann P, Klare +1 more
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Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling.
Johann P, Klare +1 more
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The binding of spin-labeled propranolol and spin-labeled progesterone by orosomucoid
Biophysical Chemistry, 1982The binding of the spin-labeled propranolol and spin-labeled progesterone to human orosomucoid has been studied as a function of temperature by electron spin resonance (ESR) techniques. At 20 degrees C the association constants are 1.9 x 10(6) and 4.9 x 10(5) M-1, respectively. In each case, the binding is competitive with unlabeled ligand. Above about
T L, Kirley, E D, Sprague, H B, Halsall
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Vision Research, 1975
Iodoacetamide and its spin-labeled derivative in high concentration ranges (10−2−10−1M) react with unexposed SH-groups of rhodopsin in the dark. The spin-labels bound to these SH-groups are moderately immobilized. While rhodopsin is not spin-labeled with the iodoacetamide at a lower concentration (5 × 10−4 m) in the dark, photobleached rhodopsin is ...
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Iodoacetamide and its spin-labeled derivative in high concentration ranges (10−2−10−1M) react with unexposed SH-groups of rhodopsin in the dark. The spin-labels bound to these SH-groups are moderately immobilized. While rhodopsin is not spin-labeled with the iodoacetamide at a lower concentration (5 × 10−4 m) in the dark, photobleached rhodopsin is ...
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Biosynthesis of spin-labeled peptidoglycan: spin-spin interactions
Biochemistry, 1977Membrane preparations from Gaffkya homari catalyzed the in vitro biosynthesis of soluble uncross-linked spin-labeled peptidoglycan, a uniformly labeled polynitroxide, from the spin-labeled nucleotide UDP-MurNAc-Ala-DGlu-Lys(Nepsilon-2,2,5,5-tetramethyl-1-pyrrolin-1-oxyl-3-carbonyl)-DAla-DAla (I) and UDP-GlcNAc.
L S, Johnston, F C, Neuhaus
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Analytical Biochemistry, 1978
Abstract A spin-label assay for lysozyme, which is based on the enzymatic hydrolysis of spin-labeled peptidoglycan, is described. Hydrolysis of this polymer by lysozyme results in sharpening of the esr spectrum. The rate of spectral sharpening is a function of enzyme concentration. When the activities of hen egg-white and human lysozymes are compared
L S, Johnston, F C, Neuhaus
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Abstract A spin-label assay for lysozyme, which is based on the enzymatic hydrolysis of spin-labeled peptidoglycan, is described. Hydrolysis of this polymer by lysozyme results in sharpening of the esr spectrum. The rate of spectral sharpening is a function of enzyme concentration. When the activities of hen egg-white and human lysozymes are compared
L S, Johnston, F C, Neuhaus
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Quarterly Reviews of Biophysics, 1981
The spin label method developed by McConnell 15 years ago is now widely used in studies of the structure and dynamic properties of a variety of the biological systems such as proteins and protein complexes, lipids and membranes, nucleic acids, nucleoproteins, etc.The ESR spectrum of the nitroxide radcal – the spin label – is very sensitive to its ...
S G, Kamzolova, G B, Postnikova
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The spin label method developed by McConnell 15 years ago is now widely used in studies of the structure and dynamic properties of a variety of the biological systems such as proteins and protein complexes, lipids and membranes, nucleic acids, nucleoproteins, etc.The ESR spectrum of the nitroxide radcal – the spin label – is very sensitive to its ...
S G, Kamzolova, G B, Postnikova
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Biochemical and Biophysical Research Communications, 1973
The compound 2,2,6,6-tetramethyl-4-[β-N-ethyleneiminopropionyl] oxypiperidine-I-oxyl is used as a spin-label for RNA. The reaction, effected under rather mild conditions, results in 50–70 nucleotides per spin-label. The temperature dependence of the ESR spectra of spin-labeled RNA is used to estimate temperatures corresponding to the beginning of ...
S, Zavriev +3 more
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The compound 2,2,6,6-tetramethyl-4-[β-N-ethyleneiminopropionyl] oxypiperidine-I-oxyl is used as a spin-label for RNA. The reaction, effected under rather mild conditions, results in 50–70 nucleotides per spin-label. The temperature dependence of the ESR spectra of spin-labeled RNA is used to estimate temperatures corresponding to the beginning of ...
S, Zavriev +3 more
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Electron Spin Resonance: Spin Labels
1981In general biological membranes possess no intrinsic paramagnetism and hence in the unlabeled state do not give rise to an electron spin resonance (ESR) spectrum. The introduction of a stable free radical (“spin label”) thus enables one to use ESR spectroscopy to study specific environments within the membrane.
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