Results 81 to 90 of about 30,422 (298)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Aggregation of Spin Labeled Trichogin GA IV Dimers: Distance Distribution between Spin Labels in Frozen Solutions by PELDOR Data. [PDF]

, 2003
Pulsed electron-electron double-resonance (PELDOR) and CW-ESR spectroscopies were used to study the dipole-dipole interaction of spin labels in frozen glassy solutions of mono- and double-labeled analogues of the 22-residue, the head-to-tail covalent ...
Y.U. D. TSVETKOV, TONIOLO, CLAUDIO, S. OANCEA, A. D. MILOV, FORMAGGIO, FERNANDO, J. RAAP   +5 more
core   +1 more source

Reduction Resistant and Rigid Nitroxide Spin-Labels for DNA and RNA [PDF]

, 2020
Electron paramagnetic resonance (EPR) spectroscopy, coupled with site-directed spin labeling (SDSL), is a useful method for studying conformational changes of biomolecules in cells.
Snorri Th. Sigurdsson, Haraldur Y. Juliusson, Snorri Th. Sigurdsson (6606), Haraldur Y. Juliusson (8500083)   +3 more
core   +1 more source

Conformation and Membrane Topology of the N-Terminal Ectodomain of Influenza A M2 Protein

Membranes
The N-terminal ectodomain of the influenza A M2 protein is a target for universal influenza vaccine development and novel antiviral strategies. Despite the significance of this domain, it is poorly understood and most structural studies of the M2 protein
Kyra C. Roepke, Kathleen P. Howard
doaj   +1 more source

Synthesis of Nanometer Sized Bis- and Tris-trityl Model Compounds with Different Extent of Spin–Spin Coupling

Molecules, 2018
Tris(2,3,5,6-tetrathiaaryl)methyl radicals, so-called trityl radicals, are emerging as spin labels for distance measurements in biological systems based on Electron Paramagnetic Resonance (EPR).
J. Jacques Jassoy, Andreas Meyer, Sebastian Spicher, Christine Wuebben, Olav Schiemann   +4 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

The use of spin labels to map protein surfaces [PDF]

, 1993
The use of spin labels to map protein ...
Motta A., Pegna, Consonni R., Zetta L., Esposito G., MOLINARI, Henriette, Niccolai N.   +6 more
core  

Aggregation of Spin Labeled Trichogin GA IV Dimers:  Distance Distribution between Spin Labels in Frozen Solutions by PELDOR Data [PDF]

, 2016
Pulsed electron−electron double-resonance (PELDOR) and CW-ESR spectroscopies were used to study the dipole−dipole interaction of spin labels in frozen glassy solutions of mono- and double-labeled analogues of the 22-residue, the head-to-tail covalent ...
J. Raap (2729350), A. D. Milov (2729353), Yu. D. Tsvetkov (2729359), S. Oancea (2729356), F. Formaggio (1960618), C. Toniolo (1960600)   +5 more
core   +1 more source

Arterial spin labeling in neuroimaging

World Journal of Radiology, 2010
Arterial spin labeling (ASL) is a magnetic resonance imaging technique for measuring tissue perfusion using a freely diffusible intrinsic tracer. As compared with other perfusion techniques, ASL offers several advantages and is now available for routine clinical practice in many institutions. Its noninvasive nature and ability to quantitatively measure
Sasitorn, Petcharunpaisan, Joana, Ramalho, Mauricio, Castillo   +2 more
openaire   +2 more sources

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source