Results 161 to 170 of about 3,491 (191)

The effect of Clostridium tyrobutyricum Spo0A overexpression in the intestine of mice

Beneficial Microbes, 2020
Clostridium tyrobutyricum shows probiotic properties and can affect the composition of gut microbiota and regulate the intestinal immune system. Compared with other probiotics, this spore-producing bacterium shows unparalleled advantages in commercial production.
Q, Liang, J, Liu, J, Wei, J, Jia, H, Shen, W, Chen, W, Liang, B, Gao, Z, Xu, L, Zhang   +9 more
openaire   +2 more sources

Domain swapping in the sporulation response regulator Spo0A

Journal of Molecular Biology, 2000
Adaptive responses of micro-organisms, such as chemotaxis and sporulation, are governed by two-component systems consisting of sensor kinases, that interpret environmental signals, and response regulators which activate the appropriate physiological responses.
R J, Lewis, K, Muchová, J A, Brannigan, I, Barák, G, Leonard, A J, Wilkinson   +5 more
openaire   +2 more sources

Interaction surface of the Spo0A response regulator with the Spo0E phosphatase

Molecular Microbiology, 2002
SummarySpo0A~P is the essential response regulator and transcription factor for sporulation initiation in Bacillus subtilis. The phosphorylation level of Spo0A in the cell is determined by the sensor kinase activity of the phosphorelay, donating phosphoryl groups, and the antagonistic effects of dephosphorylation mediated by the Rap and Spo0E ...
Sophie J, Stephenson, Marta, Perego
openaire   +2 more sources

Spo0A, The Key Transcriptional Regulator for Entrance Into Sporulation, Is an Inhibitor of DNA Replication [PDF]

EMBO Journal, 2006
The transcription factor Spo0A is a master regulator for entry into sporulation in Bacillus subtilis and also regulates expression of the virulent B. subtilis phage phi29. Here, we describe a novel function for Spo0A, being an inhibitor of DNA replication of both, the phi29 genome and the B. subtilis chromosome. Binding of Spo0A near the phi29 DNA ends,
Margarita Salas, Wilfried J J Meijer, Daniel Muñoz-Espín   +2 more
exaly   +4 more sources

Engineering a Bifunctional Phr60-Rap60-Spo0A Quorum-Sensing Molecular Switch for Dynamic Fine-Tuning of Menaquinone-7 Synthesis in Bacillus subtilis [PDF]

ACS Synthetic Biology, 2019
Quorum sensing (QS)-based dynamic regulation has been widely used as basic tool for fine-tuning gene expression in response to cell density changes without adding expensive inducers.
Guocheng Du, Long Liu, Yaokang Wu
exaly   +2 more sources

Suppressors of a spo0A missense mutation and their effects on sporulation in Bacillus subtilis

Biochimie, 1992
The spo0A gene product of Bacillus subtilis is a transcriptional regulator that is required for the initiation of sporulation. It has not been possible to isolate mutations that suppress the sporulation defect caused by spo0A null mutations. We describe the isolation and characterization of mutations that suppress the severe sporulation defect caused ...
A D, Grossman, T, Lewis, N, Levin, R, DeVivo   +3 more
openaire   +2 more sources

Assay of Transcription Modulation by Spo0A of Bacillus subtilis

2003
Publisher Summary This chapter aims to describe the methods used to purify Spo0A and some of the techniques used to study the mechanism by which Spo0A modulates transcription. Methods that are used help in developing the study of the stimulation of spoIIG transcription as an example.
Steve D Seredick, Barbara M Turner, George B Spiegelman   +2 more
openaire   +1 more source

A genetic analysis of Spo0A structure and function.

Research in microbiology, 1992
Mutations that enhanced sporulation in the presence of high concentrations of key nutrients (coi mutations) included at least four lesions within the phosphoacceptor domain of spo0A, a member of the response regulator family of "two-component" signal transduction proteins.
B D, Green, G, Olmedo, P, Youngman
openaire   +1 more source

SPO0A repression of transcription from the bacillus subtilis AbrB promoter is mediated by SPO0A tethering RNA polymerase to the promoter region

2009
Bacillus subtilis forms an endospore as a response to nutritional stress. Spore formation produces only one progeny cell, therefore it is advantageous for cells to avoid sporulating if other mechanisms for survival are available. The abrB gene product is involved in repressing sporulation. Transcription of abrB is repressed by the SpoOA protein.
openaire   +1 more source

Two-Vector Assay as a Tool for Examining Spo0A Gene Transcription Regulation

Journal of Microbiological Methods, 2002
We have modified an assay using two compatible vectors that coexist in Escherichia coli cells and applied it in the investigation of the transcriptional activity of Spo0A, a key regulator of sporulation in Bacillus subtilis. We have chosen the promoters of the Spo0A dependent genes, spoIIE and spoIIA, involved in sporulation, in order to study the ...
Imrich Barák
exaly   +3 more sources