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Gel-Free Proteomic Identification of the Bacillus subtilis Insoluble Spore Coat Protein Fraction

Proteomics, 2011
Species from the genus Bacillus have the ability to form endospores, dormant cellular forms that are able to survive heat and acid preservation techniques commonly used in the food industry.
Stanley Brul, Wishwas R Abhyankar, Chris G. de Koster   +2 more
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Studies of the spore coats of fungi I. Isolation and composition of the spore coats of Aspergillus oryzae

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides, 1964
Spore coats of Aspergillus oryzae have been mechanically isolated. They exhibited a complex chemical composition consisting of polysaccharide (mannose, glucose, galactose and glucosamine), phosphate, protein and nucleic acid. Spore coats were partially hydrolyzed by the lytic enzyme from Bacillus circulans, and glucose, laminaribiose and other unknown ...
K, HORIKOSHI, S, IIDA
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Formation of the Dictyostelium spore coat

Developmental Genetics, 1990
AbstractThe spore coat forms as a rigid extracellular wall around each spore cell during culmination. Coats purified from germinated spores contain multiple protein species and an approximately equal mass of polysaccharide, consisting mostly of cellulose and a galactose/N‐acetyl‐galactosamine polysaccharide (GPS).
C M, West, G W, Erdos
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Preparation of spore coats ofFusarium culmorum

Experientia, 1966
Se especifican las condiciones optimas para la preparation de paredes celulares de macroconidios deFusarium culmorum, utilizando el desintegrador de Mickle y se demuestra la pureza de las preparaciones mediante su examen al microscopio de fase y electronico.
I, García Acha, J P, Ballesta, M J, Aguirre, J R, Villanueva   +3 more
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Germinability of coat-lacking spores of Bacillusmegaterium

Biochemical and Biophysical Research Communications, 1985
Upon treatment with acid, the germinability of both intact and coat-lacking spores of Bacillus megaterium ATCC 19213 exhibited similar features. Namely, when the spores previously germinated by alanine in the presence of phosphate buffer were converted to H-spores by treatment with nitric acid, germination proceeded at a very low speed in a same ...
Y, Nakatani, K, Tani, M, Imagawa, T, Nishihara, M, Kondo   +4 more
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Properties of Bacillus cereus spore coat mutants

Journal of Bacteriology, 1975
Two classes of spore mutants have been selected in Bacillus cereus T, those producing lysozyme-sensitive spores, and those producing spores dependent upon lysozyme for germination. One mutant from each class was studied in detail and found to have defective packing of the spore coat layers.
A I, Aronson, P C, Fitz-James
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The spore coat of a fucosylation mutant in Dictyostelium discoideum

Developmental Biology, 1989
Strain HL250 of Dictyostelium discoideum cannot convert GDP-mannose to GDP-fucose, resulting in an inability to fucosylate protein. This affects a group of proteins which are normally fucosylated intracellularly and then secreted via prespore vesicles to become part of the outer lamina of the spore coat.
B, Gonzalez-Yanes, R B, Mandell, M, Girard, S, Henry, O, Aparicio, M, Gritzali, R D, Brown, G W, Erdos, C M, West   +8 more
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Changes in the hydrophobic characteristics of Clostridium perfringens spores and spore coats by heat

Canadian Journal of Microbiology, 1987
The hydrophobic characteristics of Clostridium perfringens NCTC 8679 spores were demonstrated by adherence to toluene in a toluene–aqueous partition system. Spores and spore coat preparations were hydrophobic. Vegetative cells and spores extracted with a dithiothreitol – sodium dodecyl sulfate treatment known to remove spore coats were not hydrophobic.
S E, Craven, L C, Blankenship
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Enzyme‐driven bacillus spore coat degradation leading to spore killing

Biotechnology and Bioengineering, 2013
ABSTRACTThe bacillus spore coat confers chemical and biological resistance, thereby protecting the core from harsh environments. The primarily protein‐based coat consists of recalcitrant protein crosslinks that endow the coat with such functional protection.
Ruchir V, Mundra, Krunal K, Mehta, Xia, Wu, Elena E, Paskaleva, Ravi S, Kane, Jonathan S, Dordick   +5 more
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Proteins of the Spore Core and Coat

2014
Abundant proteins found within the spore core (the small, acid-soluble proteins, or SASP) as well as surrounding the spore (the coat proteins) help to protect the spore from these assaults such as extremes of temperature, radiation, desiccation, and attack by a wide variety of toxic molecules. The SASP and coat proteins have been studied for many years,
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