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A Simplified Bacterial Spore Stain
Stain Technology, 1950A simplification of the Schaeffer-Fulton spore stain for bacteria is presented. It is shown that omission of the heating step during staining with malachite green resulted in spore stains as good as when the heat was applied. The simplified procedure involves (1) heat fixation of the smear by 20 passages through the flame, (2) staining with saturated ...
J W, BARTHOLOMEW, T, MITTWER
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Splitting Line of Bacterial Spore
Nature, 1967THE mechanisms involved in the germination of bacterial spores have been studied extensively from the biochemical and morphological points of view11,2. Details of morphological changes occurring during germination, however, have not yet been demonstrated clearly, It is not known whether or not, when a new vegetative cell emerges from the spore, the ...
Y, Hiragi, K, Iijima, H, Kadota
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Biosynthesis of bacterial spore coats
Journal of Molecular Biology, 1968Abstract The biochemical events associated with the assembly of bacterial spore coat proteins have been studied. Pulse-chase experiments using a variety of labeled amino acids have shown that coat precursor protein synthesis begins at the cessation of growth when sporulation commences (early forespore), but several hours prior to the appearance of ...
A I, Aronson, P C, Fitz-James
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The underlying mechanism of bacterial spore germination: An update review.
Comprehensive Reviews in Food Science and Food Safety, 2023Bacterial spores are highly resilient and universally present on earth and can irreversibly enter the food chain to cause food spoilage or foodborne illness once revived to resume vegetative growth.
Fengzhi Lyu +7 more
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Germination of Single Bacterial Spores
Journal of Bacteriology, 1969Changes in refractility and optical density occurring in individual spores of Bacillus cereus T and B. megaterium QM B1551 during germination were investigated by use of a Zeiss microscope photometer.
T, Hashimoto, W R, Frieben, S F, Conti
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THE GENETICS OF BACTERIAL SPORE GERMINATION
Annual Review of Microbiology, 1990INTRODUCTION 531 SPORE FOR/elATION AND STRUCTURE 532 SPORE GERMINATION 535 GERMINATION GENES AND THEIR ANALYSIS 538 Characteriization of Mutants 538 Genes Involved in the L-alanine Germination Pathway 540 Genes Involved in the Alternative (AGFK) Pathway 542 Genes Common to Both Germination Pathways 543 Genes lnvolved in Spore Structure
A, Moir, D A, Smith
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Heat Injury of Bacterial Spores
1978Publisher Summary This chapter discusses the heat injury of bacterial spores. Heat injury of bacterial spores, like injury of vegetative cells, is characterized by the inability of the injured spores, as a result of sublethal damage suffered during heating, to develop visible signs of growth under conditions suitable or even optimal for unheated ...
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Bacterial spore germination and protein mobility
Trends in Microbiology, 2003Fluorescence recovery after photobleaching (FRAP) of green fluorescent protein (GFP) has been used to report on protein mobility in single spores. Proteins found in dormant Bacillus spores are not mobile; however, mobility is restored when germination occurs and the core rehydrates.
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