Results 41 to 50 of about 1,335,683 (287)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Characterization of Micro-Hole Quality in Alumina Ceramics by Picosecond Laser Ring-Cut Drilling

Machines
In this study, a novel picosecond laser ring-cut drilling method was employed to drill holes in alumina ceramics. The morphology, dimensions, taper angle, and heat-affected zone (HAZ) of the resultant micro-holes were systematically characterized under ...
Wanqi Zhang, Linzheng Ye, Xijing Zhu, Shida Chuai, Peide Liu   +4 more
doaj   +1 more source

Crack imaging by scanning laser-line thermography and laser-spot thermography [PDF]

, 2011
The thermographic images of laser-heated spots or lines are perturbed by nearby cracks, providing NDE techniques for crack detection. Scanning with a laser line, rather than a laser spot, results in a substantial reduction in inspection time.
Almond, Darryl P, D A S Rees, Rees, D Andrew S, D P Almond, Li, T, T Li   +5 more
core   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Measurement of focal light spot at single-photon level with silicon photomultipliers

Scientific Reports, 2022
Focal spot (light spot) at single-photon level have important applications in many fields. This report demonstrates a method for measuring focal spot size at the single-photon level indirectly.
Guoqing Zhang, Yaxian Yang, Chen Zhang, Xinyue Cao, Lina Liu, Lianbi Li, Xiaoxiang Han   +6 more
doaj   +1 more source

Ultrasonic hand tool allows convenient scanning of spot welds [PDF]

, 1966
Small, portable, electrically powered hand tool, coupled with auxiliary ultrasonic equipment, allows convenient scanning of spot welds for ...
Mitchell, D. K.
core  

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source