Results 71 to 80 of about 1,335,683 (287)

Scanned Proton Beam Performance and Calibration of the Shanghai Advanced Proton Therapy Facility

MethodsX, 2019
The Shanghai Advanced Proton Therapy facility (SAPT) is a hospital-based facility that began construction in December of 2014 with commissioning of the first scanned proton beam line starting in October of 2017.
Hang Shu, Chongxian Yin, Haiyang Zhang, Ming Liu, Manzhou Zhang, Liying Zhao, Kecheng Chu, Xiaolei Dai, Michael F. Moyers   +8 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Experimental validation of time-driven proton PBS FLASH delivery on a clinical proton therapy system

Physics and Imaging in Radiation Oncology
Background and purpose: Clinical proton pencil beam scanning systems use dose-driven delivery, limiting operation at ultra-high dose rates required for FLASH radiotherapy.
Dawid Krzempek, Jan Gajewski, Hubert Jabłoński, Anna Spaleniak, Wiktor Komenda, Konrad Guguła, Michał Kamiński, Michał Hankiewicz, Tomasz Pasiecznik, Krzysztof Suder, Leszek Malinowski, Tomasz Nowak, Natalia Mojżeszek, Marta Bałamut, Paweł Rogalski, Renata Kopeć, Marzena Rydygier   +16 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Sub-wavelength focusing of high intensities in microfibre tips

Sub-wavelength efficient intensity confinement has been demonstrated in nanostructured optical microfibre tips. Focus Ion Beam (FIB) milling was used to nanostructure gold-coated optical microfibre tips and form apertures at the apex.
Ding, M., Fenwick, O., Cacialli, F., Ou, J-Y., Sessions, N.P., Xu, F., Jung, Y., Kou, J.-L., Brambilla, G., Di Stasio, F.   +9 more
core   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

An algorithm for super-resolved reconstruction of single-photon emitter locations from g(2)(0) maps

Results in Optics
Single-photon sources are vital for emerging quantum technologies. In particular, Nitrogen-Vacancy (NV) centers in diamond are promising due to their room-temperature stability, long spin coherence, and compatibility with nanophotonic structures.
Sonali Gupta, Amit Kumar, Vikas S. Bhat, Sushil Mujumdar   +3 more
doaj   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source