Results 241 to 250 of about 2,136,124 (276)

Stable Isotope Probing and Metagenomics

2014
Two promising culture-independent approaches that have been employed to assess the function and metabolic potential of uncultivated microorganisms are stable isotope probing (SIP) and metagenomics. This chapter discusses the methodology of metagenomics within the context of DNA stable isotope probing (DNA-SIP), and provides a description of the ...
Lee J. Pinnell, Trevor C. Charles, Josh D. Neufeld   +2 more
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Protein Stable Isotope Probing

2014
The chapter on protein stable isotope probing (protein-SIP) focuses on cultivation conditions, extraction of proteins, different protein-separation techniques, methods of mass spectrometry (MS) analysis, and the calculation of stable isotope incorporation levels.
Nico Jehmlich, Jana Seifert, Martin Taubert, Frank Schmidt, Carsten Vogt, Hans-Hermann Richnow, Martin von Bergen   +6 more
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RNA Stable Isotope Probing (RNA-SIP)

2019
Stable isotope probing is a combined molecular and isotopic technique used to probe the identity and function of uncultivated microorganisms within environmental samples. Employing stable isotopes of common elements such as carbon and nitrogen, RNA-SIP exploits an increase in the buoyant density of RNA caused by the active metabolism and incorporation ...
Noor-Ul-Huda, Ghori, Benjamin, Moreira-Grez, Paton, Vuong, Ian, Waite, Tim, Morald, Michael, Wise, Andrew S, Whiteley   +6 more
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Stable Isotopes as Metabolic Probes for Nutritional Studies in Neonates

Clinics in Perinatology, 1995
Metabolic and nutritional studies with stable isotope tracers have proved valuable in the human newborn. This technique is acceptable ethically, because it does not expose the infant to radioactivity, and it is sensitive enough for the analysis of very small samples.
M, Yudkoff, I, Nissim
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Identification of cellulolytic bacteria in soil by stable isotope probing

Environmental Microbiology, 2006
Summary Plant residues, mainly made up of cellulose, are the largest fraction of organic carbon material in terrestrial ecosystems. Soil microorganisms are mainly responsible for the transfer of this carbon to the atmosphere, but their contribution is not accurately known.
Haichar, Feth El Zahar, Achouak, Wafa, Christen, Richard, Heulin, Thierry, Marol, Christine, Marais, Marie-France, Mougel, Christophe, Ranjard, Lionel, Balesdent, Jérôme, Berge, Odile   +9 more
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Profiling of Active Microorganisms by Stable Isotope Probing—Metagenomics

Methods in Molecular Biology, 2019
Stable isotope probing (SIP) provides researchers a culture-independent method to retrieve nucleic acids from active microbial populations performing a specific metabolic activity in complex ecosystems. In recent years, the use of the SIP method in microbial ecology studies has been accelerated.
Ozge Eyice, Eileen Kröber
exaly   +4 more sources

Stable isotope probing of carbon flow in the plant holobiont

Current Opinion in Biotechnology, 2016
Microbial communities associated with a plant host, constituting a holobiont, affect the physiology and growth of the plant via metabolites that are mainly derived from their photosynthates. The structure and function of active microbial communities that assimilate root exudates can be tracked by using stable isotope probing (SIP) approaches.
Haichar-Baya, Feth-El-Zahar, Heulin, Thierry, Guyonnet, Julien P., Achouak, Wafa   +3 more
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Stable isotopes as probes for the metabolism of acetanilide in man and the rat

Biological Mass Spectrometry, 1988
Acetanilide with the three hydrogens of the acetyl group replaced by deuterium was administered to 9 human volunteers (50 mg p.o.) and 5 male Sprague-Dawley rats (10 mg kg-1 p.o. and 100 mg kg-1 p.o.) and urine samples collected for 8 and 48 h, respectively.
J D, Baty, R M, Lindsay, W R, Fox, R G, Willis   +3 more
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In Situ Stable Isotope Probing of Methanogenic Archaea in the Rice Rhizosphere

Science, 2005
Microorganisms living in anoxic rice soils contribute 10 to 25% of global methane emissions. The most important carbon source for CH 4 production is plant-derived carbon that enters soil as root exudates and debris. Pulse labeling of rice plants with 13
Lu, Y., Conrad, R.
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Stable Isotope Probing and the Human Gut

2014
This chapter describes the recent developments that have started to shed some light on the fermentative capacity of the colonic microbiota and its contribution to health and disease. The combined use of stable isotope probing (SIP) with analytical, gastroenterological, nanotechnological, and phylogenetic microarray techniques is highlighted in the ...
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