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Stable Isotope Probing Techniques and Bioremediation
2014This chapter summarizes the state of the art for applications of stable isotope probing (SIP) to bio-degradation and bioremediation research. SIP is one of the many emerging tools of inquiry used by environmental microbiologists. The goals of this chapter are to catalog and analyze trends exhibited by the majority of studies published to date that are ...
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Stable Isotope Probing of Microorganisms in Environmental Samples with H218O
2019We describe a protocol for investigating microbial growth in environmental samples via stable isotope probing (SIP) with H218O. Water is a universal substrate for all microorganisms and replication is required for DNA to become labeled with 18O. By measuring how much the DNA of each taxon becomes enriched with 18O when an environmental sample is ...
Egbert, Schwartz +3 more
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DNA stable Isotope Probing and Gene Mining
2014This chapter reviews the state of the art in isolation of new and valuable industrial enzymes from natural biodiversity, and discusses the strengths and weaknesses of each approach and shows how results with DNA stable isotope probing (DNA-SIP) indicate that this technique has substantial promise for improving the effectiveness of strategies for ...
Hélène Moussard +2 more
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Microbial Taxon-Specific Isotope Incorporation with DNA Quantitative Stable Isotope Probing
2019Quantitative stable isotope probing (qSIP) measures rates of taxon-specific element assimilation in intact microbial communities, utilizing substrates labeled with a heavy isotope.The laboratory protocol for qSIP is nearly identical to that for conventional stable isotope probing, with two key additions: (1) in qSIP, qPCR measurements are conducted on ...
Brianna K, Finley +7 more
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Application of 13C-Stable Isotope Probing to Identify RDX-Degrading Microorganisms in Groundwater
Environmental Pollution, 2013Kung-Hui Chu +2 more
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Unraveling Uncultivable Pesticide Degraders via Stable Isotope Probing (SIP)
Critical Reviews in Biotechnology, 2018Bo Jiang, Dayi Zhang, Naifu Jin
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