A culture-independent approach to unravel uncultured bacteria and functional genes in a complex microbial community [PDF]
, 2012 Most microorganisms in nature are uncultured with unknown functionality. Sequence-based metagenomics alone answers 'who/what are there?' but not 'what are they doing and who is doing it and how?'.
Zhou, Q. +47 more
core +2 more sources
Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies
Molecules, 2021 RNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions. NMR spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution.
Lukasz T. Olenginski +3 more
doaj +1 more source
Differential Activity of Coexisting Prochlorococcus Ecotypes
Frontiers in Marine Science, 2019 The abundance and widespread distribution of the unicellular cyanobacterium Prochlorococcus is attributed to its extensive genetic diversity. At least twelve distinct clades, or ecotypes, of Prochlorococcus have been discovered so far, and follow ...
Anne W. Thompson +2 more
doaj +1 more source
Emerging experimental and computational methods for studying redox‐regulated structural transitions
FEBS Letters, EarlyView.Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
¹⁵N stable isotope probing of pulp and paper wastewaters [PDF]
, 2008 Stable isotope probing (SIP) is an established technique that can be applied to identify the metabolically active micro-organisms within a microbial population.
Addison, Sarah Louise
core
AgricultureSoil microorganisms are responsible for natural glyphosate dissipation. However, previous studies have largely focused on bulk soils, and the underlying microscale mechanisms remain poorly resolved.
Shuwen Luo +7 more
doaj +1 more source
Smaller is better: nanobodies meet NMR
FEBS Letters, EarlyView.Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley +1 more source
Analyzing the functionality of the human intestinal microbiota by stable isotope probing [PDF]
, 2010 Key words: gut bacteria, dietary carbohydrates, digestion, RNA-SIP, TIM-2, HITChip, human trial The human gastro-intestinal (GI) tract comprises a series of complex and dynamic organs ranging from the stomach to the distal colon, which harbor immense ...
Kovatcheva, P.P.
core
Stable oxygen and carbon isotope compositional fields for skeletal and diagenetic components in New Zealand Cenozoic nontropical carbonate sediments and limestones: A synthesis and review [PDF]
, 1996 The stable oxygen isotope composition (δ18O) of a precipitated carbonate depends mainly on the isotope composition, salinity, and temperature of the host fluid, whereas the stable carbon isotope composition (δ13C) reflects the source of CO2 for ...
Nelson, Campbell S. +3 more
core +1 more source
Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment
Molecular Oncology, EarlyView.This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley +1 more source