Results 21 to 30 of about 2,136,124 (276)

RNA-Stable Isotope Probing v6 [PDF]

, 2021
The following protocol describes how to perform an RNA-Stable Isotope Probing experiment. The scope of this protocol only covers the parts involving separating labelled RNA from unlabelled RNA using ultracentrifugation in a caesium trifluoroacetate density gradient and downstream quantification to evaluate whether the labelling and separation of the ...
Roey Angel, Eva Petrova, Ana Lara
openaire   +1 more source

Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics

Biotechnology for Biofuels and Bioproducts, 2023
The biogas produced through anaerobic digestion (AD) of renewable feedstocks is one of the promising alternatives to replace fossil-derived energy. Even though lignocellulosic biomass is the most abundant biomass on earth, only a small fraction is being ...
Jan Struckmann Poulsen, Williane Vieira Macêdo, Torben Bonde, Jeppe Lund Nielsen   +3 more
doaj   +1 more source

Impact of plants on the diversity and activity of methylotrophs in soil

Microbiome, 2020
Background Methanol is the second most abundant volatile organic compound in the atmosphere, with the majority produced as a metabolic by-product during plant growth.
Michael C. Macey, Jennifer Pratscher, Andrew T. Crombie, J. Colin Murrell   +3 more
doaj   +1 more source

DNA Stable-Isotope Probing (DNA-SIP) [PDF]

Journal of Visualized Experiments, 2010
DNA stable-isotope probing (DNA-SIP) is a powerful technique for identifying active microorganisms that assimilate particular carbon substrates and nutrients into cellular biomass. As such, this cultivation-independent technique has been an important methodology for assigning metabolic function to the diverse communities inhabiting a wide range of ...
Eric A, Dunford, Josh D, Neufeld
openaire   +2 more sources

Bacterial Necromass Is Rapidly Metabolized by Heterotrophic Bacteria and Supports Multiple Trophic Levels of the Groundwater Microbiome

Microbiology Spectrum, 2022
Pristine groundwater is a highly stable environment with microbes adapted to dark, oligotrophic conditions. Input events like heavy rainfalls can introduce the excess particulate organic matter, including surface-derived microorganisms, thereby ...
Patricia Geesink, Martin Taubert, Nico Jehmlich, Martin von Bergen, Kirsten Küsel   +4 more
doaj   +1 more source

RNA-Stable Isotope Probing v5 [PDF]

, 2021
The following protocol describes how to perform an RNA-Stable Isotope Probing experiment. The scope of this protocol only covers the parts involving separating labelled RNA from unlabelled RNA using ultracentrifugation in a caesium trifluoroacetate density gradient and downstream quantification to evaluate whether the labelling and separation of the ...
Roey Angel, Eva Petrova, Ana Lara
openaire   +1 more source

Microbial ecology of arsenic-mobilizing Cambodian sediments: Lithological controls uncovered by stable-isotope probing [PDF]

, 2014
Microbially mediated arsenic release from Holocene and Pleistocene Cambodian aquifer sediments was investigated using microcosm experiments and substrate amendments.
Rizoulis, A, Cooke, D A, Richard D. Pancost, Rizoulis, Athanasios, Cooke, David, Pancost, Richard D., David A. Polya, Pancost, R D, Lloyd, Jonathan R., Sanguin, Herve, Athanasios Rizoulis, Lloyd, J R; id_orcid, Hervé Sanguin, Sanguin, H, Cooke, David A., Polya, David, Marina Héry, Jonathan R. Lloyd, Pancost, Richard, Héry, Marina, Lloyd, Jonathan, Hery, M, Polya, David A., David A. Cooke, Polya, David A; id_orcid   +24 more
core   +1 more source

Stable Isotope Probing Identifies Bacterioplankton Lineages Capable of Utilizing Dissolved Organic Matter Across a Range of Bioavailability

Frontiers in Microbiology, 2020
Bacterioplankton consume about half of the dissolved organic matter (DOM) produced by phytoplankton. DOM released from phytoplankton consists of a myriad of compounds that span a range of biological reactivity from labile to recalcitrant.
Shuting Liu, Nicholas Baetge, Jacqueline Comstock, Keri Opalk, Rachel Parsons, Elisa Halewood, Chance J. English, Stephen Giovannoni, Luis M. Bolaños, Craig E. Nelson, Kevin Vergin, Craig A. Carlson   +11 more
doaj   +1 more source

Stable Isotope Probing-RNA Strategy to Study Plant/Fungus Interactions

, 2023
The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment.
Vandenkoornhuyse, Philippe, Duhamel, Marie, Lê Van, Amandine, Quaiser, Achim   +3 more
core   +1 more source

Polyphosphate Dynamics in Cable Bacteria

Frontiers in Microbiology, 2022
Cable bacteria are multicellular sulfide oxidizing bacteria that display a unique metabolism based on long-distance electron transport. Cells in deeper sediment layers perform the sulfide oxidizing half-reaction whereas cells in the surface layers of the
Nicole M. J. Geerlings, Michiel V. M. Kienhuis, Silvia Hidalgo-Martinez, Silvia Hidalgo-Martinez, Renee Hageman, Diana Vasquez-Cardenas, Jack J. Middelburg, Filip J. R. Meysman, Filip J. R. Meysman, Lubos Polerecky   +9 more
doaj   +1 more source