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Quantitative microbial ecology through stable isotope probing [PDF]
Bacteria grow and transform elements at different rates, yet quantifying this variation in the environment is difficult. Determining isotope enrichment with fine taxonomic resolution after exposure to isotope tracers could help, but there are few suitable techniques.
Bruce A Hungate +11 more
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Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of organisms that incorporate particular substrates are identified without the prerequisite of cultivation. Stable-isotope-labeled carbon (13C) or nitrogen (15N) sources are assimilated into microbial biomass of environmental samples ...
Neufeld, Josh D. +6 more
openaire +5 more sources
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA ...
Dumont, Marc, Hernández, Marcela
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Novel Phenanthrene-Degrading Bacteria Identified by DNA-Stable Isotope Probing. [PDF]
Microorganisms responsible for the degradation of phenanthrene in a clean forest soil sample were identified by DNA-based stable isotope probing (SIP).
Longfei Jiang +4 more
doaj +2 more sources
Evaluating the accuracy of DNA stable isotope probing [PDF]
Originality-Significance Statement By combining DNA Stable Isotope Probing (DNA-SIP) with multiplexed high throughput DNA sequencing (HTS-DNA-SIP), it is now possible to identify patterns of isotope incorporation for thousands of microbial taxa.
Youngblut, Nicholas D. +1 more
openaire +2 more sources
Applications of DNA-Stable Isotope Probing in Bioremediation Studies
DNA-stable isotope probing, a method to identify active microorganisms without the prerequisite of cultivation, has been widely applied in the study of microorganisms involved in the degradation of environmental pollutants. Recent advances and technique considerations in applying DNA-SIP in bioremediation are highlighted.
Chen, Y., Vorha, J., Murrell, J. C.
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Stable Isotope Probing of Hydrocarbon-Degraders
Microbes govern a plethora of biogeochemical reactions in natural systems. Especially, they are crucial for the degradation of hydrocarbons and contaminants under both aerobic and anaerobic conditions. Only in the last 1–2 decades, researchers have
T. Lueders, Lüders, T.
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Single-cell stable isotope probing in microbial ecology. [PDF]
Abstract Environmental and host-associated microbiomes are typically diverse assemblages of organisms performing myriad activities and engaging in a network of interactions that play out in spatially structured contexts. As the sum of these activities and interactions give rise to overall microbiome function, with important consequences ...
Alcolombri U +3 more
europepmc +6 more sources
Bacterial Consumption of T4 Phages
The bacterial consumption of viruses not been reported on as of yet even though bacteria feed on almost anything. Viruses are widely distributed but have no acknowledged active biocontrol.
Jean-Jacques Godon +6 more
doaj +1 more source
HTSSIP: An R package for analysis of high throughput sequencing data from nucleic acid stable isotope probing (SIP) experiments. [PDF]
Combining high throughput sequencing with stable isotope probing (HTS-SIP) is a powerful method for mapping in situ metabolic processes to thousands of microbial taxa.
Nicholas D Youngblut +2 more
doaj +1 more source

