Results 131 to 140 of about 1,347 (156)

Protocol for Performing Protein Stable Isotope Probing (Protein-SIP) Experiments

Springer Protocols, 2016
Nico Jehmlich, Martin Von Bergen, Von Bergen Martin   +2 more
exaly   +3 more sources

Raman stable isotope probing (SIP): A novel bio-analytical tool

TrAC - Trends in Analytical Chemistry
S P Singh, Jiro Karlo
exaly   +2 more sources

Protein‐based stable isotope probing (protein‐SIP) in functional metaproteomics

Mass Spectrometry Reviews, 2012
AbstractThe community phenotype as the sum of molecular functions of organisms living in consortia strongly depends on interactions within these communities. Therefore, the analyses of the most significant molecules in terms of the phenotype, the proteins, have to be performed on samples without disrupting the meta‐species environment.
Jana, Seifert, Martin, Taubert, Nico, Jehmlich, Frank, Schmidt, Uwe, Völker, Carsten, Vogt, Hans-Hermann, Richnow, Martin, von Bergen   +7 more
openaire   +2 more sources

Metagenomics Combined with Stable Isotope Probe (SIP) for the Discovery of Novel Dehalogenases Producing Bacteria

Bulletin of Environmental Contamination and Toxicology, 2020
Halogenated compounds are one of the largest groups of environmental-hazardous chemicals. The removal of the halogen atom from the substrate is possible by the catalytic activity of a type of enzyme called dehalogenase. Hydrolytic dehalogenases are suggested to be a good biodegradation catalyst for halogenated compounds with potential bioremediation ...
Hemen Sarma, Sanket J. Joshi
openaire   +2 more sources

Insights into the fate of a 13C labelled phenol pulse for stable isotope probing (SIP) experiments

Journal of Microbiological Methods, 2007
Stable isotope probing (SIP) using DNA or RNA as a biomarker has proven to be a useful method for attributing substrate utilisation to specific microbial taxa. In this study we followed the transfer of a (13)C(6)-phenol pulse in an activated sludge micro-reactor to examine the resulting distribution of labelled carbon in the context of SIP. Most of the
Manefield, Mike, Griffiths, Rob, McNamara, Niall P., Sleep, Darren, Ostle, Nick, Whiteley, Andrew   +5 more
openaire   +3 more sources

RNA-Based Stable Isotope Probing (RNA-SIP) in the Gut Environment

2019
The RNA-SIP technology allows for linking the structure and function of complex microbial communities, that is, the identification of microbial key players involved in distinct degradation and assimilation processes under in situ conditions. Being dependent on RNA, this technique is particularly suited for environments with high numbers of very active,
Weis, Severin, Schnell, Sylvia, Egert, Markus   +2 more
openaire   +3 more sources

RNA-based stable isotope probing (RNA-SIP) to unravel intestinal host-microbe interactions

Methods, 2018
The RNA-SIP technology, introduced into molecular microbial ecology in 2002, is an elegant technique to link the structure and function of complex microbial communities, i.e. to identify microbial key-players involved in distinct degradation and assimilation processes under in-situ conditions.
Egert, Markus, Weis, Severin, Schnell, Sylvia   +2 more
openaire   +3 more sources

HT-SIP: A semi-automated Stable Isotope Probing pipeline identifies interactions in the hyphosphere of arbuscular mycorrhizal fungi

2022
ABSTRACT Background Linking the identity of wild microbes with their ecophysiological traits and environmental functions is a key ambition for microbial ecologists. Of many techniques that strive to meet this goal, Stable Isotope Probing—SIP—remains the most comprehensive for ...
Erin E. Nuccio, Steven J. Blazewicz, Marissa Lafler, Ashley N. Campbell, Anne Kakouridis, Jeffrey A. Kimbrel, Jessica Wollard, Dariia Vyshenska, Robert Riley, Andy Tomatsu, Rachel Hestrin, Rex R. Malmstrom, Mary Firestone, Jennifer Pett-Ridge   +13 more
openaire   +1 more source

Incorporation of carbon and nitrogen atoms into proteins measured by protein‐based stable isotope probing (Protein‐SIP)

Rapid Communications in Mass Spectrometry, 2008
Abstract The identification of metabolically active microbial key players is fundamental for understanding the structure and functions of contaminant‐degrading communities. The metabolic activity can be analysed by feeding the microbial culture with stable‐isotope‐labelled substrates and subsequently tracing their
Nico, Jehmlich, Frank, Schmidt, Mathias, Hartwich, Martin, von Bergen, Hans-Hermann, Richnow, Carsten, Vogt   +5 more
openaire   +2 more sources

Different phenanthrene degraders between free-cell mediated and biochar-immobilization assisted soil bioaugmentation as identified by RNA-based stable isotope probing (RNA-SIP)

Science of The Total Environment, 2023
Bioaugmentation (BA) is an effective approach to remove polycyclic aromatic hydrocarbons (PAHs) from contaminated soils, and biochar is frequently used to enhance PAH degradation performance. In this study, phenanthrene (PHE) degradation behavior and active degraders in a petroleum-contaminated soil were investigated and compared between free-cell ...
Tingting, Teng, Jidong, Liang, Zijun, Wu, Pengkang, Jin, Dayi, Zhang   +4 more
openaire   +2 more sources