Results 51 to 60 of about 383,377 (258)

Recovered or Perfected: The Discourse of Chemistry in the Nineteenth-Century Revival of Stained Glass in Britain and France

19, 2020
The science of chemistry revitalized the art of stained glass in nineteenth-century Europe, recovering old techniques, developing new materials, and discovering new artistic methods.
Thea Goldring
doaj   +2 more sources

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Visual Reinterpretation of Eco-theology in Modern Church Stained Glass: The Meaning of Jesus' Ascension into Heaven [PDF]

Pharos Journal of Theology
Visuals on modern church stained glass are rarely discussed as an object of study, even though this phenomenon has crucial significance in representing theological narratives.
Irenia Jastisia, Hendri Efendi H. Hutahaean   +1 more
doaj   +1 more source

A story from letters and memoirs. Konstantin Ligsky —“anthroposophist of Dornach” [PDF]

Литературный факт, 2018
The article treats about Konstantin Ligsky, a well-known activist of the new communist regime in Russia after the 1917 revolution. In the research perspective, however, the analysis was subjected to the years 1914–1916, when Ligsky joined the ...
Diana Oboleńska
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

‘Daylight upon magic’: Stained Glass and the Victorian Monarchy

19, 2020
This article presents stained glass as an important source of material evidence for attitudes to monarchy in Victorian Britain. It argues that expressions of veneration for Victoria by different religious constituencies were not merely instinctual and ...
Michael Ledger-Lomas
doaj   +2 more sources

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source