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Cold Spring Harbor Protocols, 2022
When labeled antibodies are used to detect antigens in cells or tissues, several characteristics of an antigen can be readily determined. Most importantly, cell staining will show both the presence and subcellular localization of an antigen. Double-labeling techniques permit the simultaneous detection of two antigens, allowing comparisons of the ...
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When labeled antibodies are used to detect antigens in cells or tissues, several characteristics of an antigen can be readily determined. Most importantly, cell staining will show both the presence and subcellular localization of an antigen. Double-labeling techniques permit the simultaneous detection of two antigens, allowing comparisons of the ...
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Differential Staining of Bacteria: Flagella Stain
Current Protocols in Microbiology, 2009AbstractBacterial flagella are appendages used for motility. Their presence is a useful tool for identification and differentiation of prokaryotes. Since flagella are too thin to be seen by compound light microscopy, staining methods employ the use of a mordant (often tannic acid) to make them thick enough to see using an oil immersion objective.
Donald P, Breakwell +2 more
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Differential Staining of Bacteria: Endospore Stain
Current Protocols in Microbiology, 2009AbstractEndospore production is a very important characteristic of some bacteria, allowing them to resist adverse environmental conditions such as desiccation, chemical exposure, extreme heat, radiation, etc. The identification of endospores is also very important for the clinical microbiologist who is analyzing a patient's body fluid or tissue—there ...
Jackie, Reynolds +2 more
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2017
Historically, the diagnosis and classification of acute leukemia involved morphologic review of blasts in the peripheral blood and bone marrow smears and cytochemical staining. Cytochemical stains, which are enzymatic colorimetric reactions that occur in the cells of interest, were necessary to assign and confirm myeloid and lymphoid lineage.
Michele E, Paessler +2 more
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Historically, the diagnosis and classification of acute leukemia involved morphologic review of blasts in the peripheral blood and bone marrow smears and cytochemical staining. Cytochemical stains, which are enzymatic colorimetric reactions that occur in the cells of interest, were necessary to assign and confirm myeloid and lymphoid lineage.
Michele E, Paessler +2 more
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Preliminary Staining of Bacteria: Simple Stains
Current Protocols in Microbiology, 2009AbstractSimple staining involves directly staining the bacterial cell with a positively charged dye in order to see bacterial detail, in contrast to negative staining where the bacteria remain unstained against a dark background. Curr. Protoc. Microbiol. 15:A.3E.1‐A.3E.5. © 2009 by John Wiley & Sons, Inc.
Rita B, Moyes +2 more
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Cold Spring Harbor Protocols, 2006
INTRODUCTIONStaining yeast cells for the presence and localization of antigens has been particularly challenging because of several factors. The yeast cells are small, making the resolution of any antigen difficult; they have a thick cell wall that antibodies cannot penetrate and that is difficult to remove; and they grow in suspension, making handling
Ed, Harlow, David, Lane
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INTRODUCTIONStaining yeast cells for the presence and localization of antigens has been particularly challenging because of several factors. The yeast cells are small, making the resolution of any antigen difficult; they have a thick cell wall that antibodies cannot penetrate and that is difficult to remove; and they grow in suspension, making handling
Ed, Harlow, David, Lane
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Preliminary Staining of Bacteria: Negative Stain
Current Protocols in Microbiology, 2009AbstractNegative staining is one of the many staining techniques that can be employed for viewing of bacterial cell morphology and size. The advantages of the negative stain include the use of only one stain and the absence of heat fixation of the sample.
Rita B, Moyes +2 more
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Immunoperoxidase staining of previously stained tissue
Journal of Cutaneous Pathology, 1984Using an unlabelled antibody peroxidase‐antiperoxidase method, We have demonstrated than certain tissue antigens can be identified in tissue sections previously stained with hematoxylin and eosin. This technique is useful in identifying antigens in tissues where insufficient unstained material is available.
D, Walder, N S, Penneys, M, Nadji
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Stain–Decolorize–Stain (SDS): a new technique for multiple staining
Histochemistry and Cell Biology, 2014Multiple staining of more than one gene/antigen on a single tissue section is an indispensable tool in cell and tissue research. However, most of the available multiple staining techniques have limitations, and there has been no technique to simultaneously visualize and distinguish tissue antigens, nucleotide sequences and other chemical compounds on ...
Jing, Li, Yan, Zhou, Jiang, Gu
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