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Preliminary Staining of Bacteria: Simple Stains
Current Protocols in Microbiology, 2009AbstractSimple staining involves directly staining the bacterial cell with a positively charged dye in order to see bacterial detail, in contrast to negative staining where the bacteria remain unstained against a dark background. Curr. Protoc. Microbiol. 15:A.3E.1‐A.3E.5. © 2009 by John Wiley & Sons, Inc.
Rita B, Moyes +2 more
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Immunoperoxidase staining of previously stained tissue
Journal of Cutaneous Pathology, 1984Using an unlabelled antibody peroxidase‐antiperoxidase method, We have demonstrated than certain tissue antigens can be identified in tissue sections previously stained with hematoxylin and eosin. This technique is useful in identifying antigens in tissues where insufficient unstained material is available.
D, Walder, N S, Penneys, M, Nadji
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Preliminary Staining of Bacteria: Negative Stain
Current Protocols in Microbiology, 2009AbstractNegative staining is one of the many staining techniques that can be employed for viewing of bacterial cell morphology and size. The advantages of the negative stain include the use of only one stain and the absence of heat fixation of the sample.
Rita B, Moyes +2 more
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Differential Staining of Bacteria: Capsule Stain
Current Protocols in Microbiology, 2009AbstractBacterial capsules are composed of high‐molecular‐weight polysaccharides and/or polypeptides, and are associated with virulence and biofilm formation. Unfortunately, capsules do not stain well with crystal violet, methylene blue, or other simple stains. This unit describes two methods of capsule staining.
Donald P, Breakwell +2 more
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Differential Staining of Bacteria: Endospore Stain
Current Protocols in Microbiology, 2009AbstractEndospore production is a very important characteristic of some bacteria, allowing them to resist adverse environmental conditions such as desiccation, chemical exposure, extreme heat, radiation, etc. The identification of endospores is also very important for the clinical microbiologist who is analyzing a patient's body fluid or tissue—there ...
Jackie, Reynolds +2 more
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Differential Staining of Bacteria: Gram Stain
Current Protocols in Microbiology, 2009AbstractIn 1884, Hans Christian Gram, a Danish doctor, developed a differential staining technique that is still the cornerstone of bacterial identification and taxonomic division. This multistep, sequential staining protocol separates bacteria into four groups based on cell morphology and cell wall structure: Gram‐positive cocci, Gram‐negative cocci ...
Rita B, Moyes +2 more
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Coffee stain on textiles. Mechanisms of staining and stain removal
Journal of the American Oil Chemists' Society, 1995AbstractCoffee stains on textiles are mainly caused by the water‐soluble and acidic colored substances in coffee. The acidic nature of coffee stain has been shown by ultraviolet and visible spectroscopy of coffee as a function of pH; ion‐pair formation with a cationic surfactant and titration with Hyamine 1622 and a surfactant‐specific electrode; and ...
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Stain Technology: Progress in the Standardization of Stains: The History of Staining
Stain Technology, 1932(1932). Stain Technology: Progress in the Standardization of Stains: The History of Staining. Stain Technology: Vol. 7, No. 4, pp. 113-114.
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Cold Spring Harbor Protocols, 2022
When labeled antibodies are used to detect antigens in cells or tissues, several characteristics of an antigen can be readily determined. Most importantly, cell staining will show both the presence and subcellular localization of an antigen. Double-labeling techniques permit the simultaneous detection of two antigens, allowing comparisons of the ...
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When labeled antibodies are used to detect antigens in cells or tissues, several characteristics of an antigen can be readily determined. Most importantly, cell staining will show both the presence and subcellular localization of an antigen. Double-labeling techniques permit the simultaneous detection of two antigens, allowing comparisons of the ...
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2017
Historically, the diagnosis and classification of acute leukemia involved morphologic review of blasts in the peripheral blood and bone marrow smears and cytochemical staining. Cytochemical stains, which are enzymatic colorimetric reactions that occur in the cells of interest, were necessary to assign and confirm myeloid and lymphoid lineage.
Michele E, Paessler +2 more
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Historically, the diagnosis and classification of acute leukemia involved morphologic review of blasts in the peripheral blood and bone marrow smears and cytochemical staining. Cytochemical stains, which are enzymatic colorimetric reactions that occur in the cells of interest, were necessary to assign and confirm myeloid and lymphoid lineage.
Michele E, Paessler +2 more
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