Characterization of intestinal fibrosis in cats with chronic inflammatory enteropathy
Journal of Veterinary Internal Medicine, 2023 Background Intestinal fibrosis (IF) is commonly identified on histopathology of intestinal biopsy specimens (IBSp) from cats with chronic inflammatory enteropathy (CIE) however, its clinical relevance is unknown. Objectives Characterize and determine the
Yuvani Bandara +3 more
doaj +1 more source
Morphological analysis of CDC2 and glycogen synthase kinase 3β phosphorylation as markers of g2 → m transition in glioma. [PDF]
, 2011 G2 → M transition is a strategic target for glioma chemotherapy. Key players in G2 → M transition include CDC2 and glycogen synthase kinase 3β (GSK3β), which are highly regulated by posttranslational phosphorylation.
Otero, José Javier, Tihan, Tarik
core +3 more sources
Labeling Extracellular Vesicles for Nanoscale Flow Cytometry
Scientific Reports, 2017 Extracellular vesicles (EVs), including exosomes and microvesicles, are 30–800 nm vesicles that are released by most cell types, as biological packages for intercellular communication. Their importance in cancer and inflammation makes EVs and their cargo
A. Morales-Kastresana +16 more
semanticscholar +1 more source
Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections
bioRxiv, 2017 Multiplexing, labeling for multiple immunostains in the very same cell or tissue section in situ, has raised considerable interest. The methods proposed include the use of labeled primary antibodies, spectral separation of fluorochromes, bleaching of the
M. Bolognesi +6 more
semanticscholar +1 more source
Frontiers in Medicine, 2022 Several cases of pigmented mammary Paget’s disease (PMPD) mimicking cutaneous malignant melanoma have been reported. In these cases, the tumor cells are colonized by melanocytes, particularly with the presence of a population of melanocytes staining for ...
Shijia Rao +7 more
doaj +1 more source
Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery [PDF]
, 2013 Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to
Attila Bokros +10 more
core +2 more sources
Noninvasive in vivo tracking of mesenchymal stem cells and evaluation of cell therapeutic effects in a murine model using a clinical 3.0 T MRI [PDF]
, 2013 Cardiac cell therapy with mesenchymal stem cells (MSCs) represents a promising treatment approach for endstage heart failure. However, little is known about the underlying mechanisms and the fate of the transplanted cells. The objective of the presented
Bovenschulte, Henning +11 more
core +1 more source
BioTechniques, 2001 Labeling of proteins with SYPRO® Orange, SYPRO Red, and SYPRO Ruby after 2-D polyacrylamide gel electrophoresis (PAGE) using plastic-backed immobilized pH gradient (IPG) strips and precast SDS polyacrylamide gels was tested.
Klaus Herick +3 more
doaj +1 more source
Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]
, 2020 BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F. +2 more
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Magnetic Particle Imaging tracks the long-term fate of in vivo neural cell implants with high image contrast. [PDF]
, 2015 We demonstrate that Magnetic Particle Imaging (MPI) enables monitoring of cellular grafts with high contrast, sensitivity, and quantitativeness. MPI directly detects the intense magnetization of iron-oxide tracers using low-frequency magnetic fields. MPI
Conolly, Steven +7 more
core +1 more source