Results 51 to 60 of about 359,491 (304)

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Evaluation of different decalcifying agents on histochemical and immunohistochemical staining properties of canine osseous tissue [PDF]

Medical Laboratory Journal
Background: The purpose of tissue processing is to strengthen the tissue and place it in a suitable environment for slicing without causing damage.
Reyhaneh Shafieian, Alireza Ebrahimzadeh-Bideskan, Esmail Ranjbar   +2 more
doaj  

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Sensitivity of Diagnostic Tests for Dry Eye in Patients with Blepharospasm

Acta Clinica Croatica, 2017
The aim of the study was to evaluate diagnostic tests for keratoconjunctivitis sicca (Schirmer test, tear break-up time (TBUT) test, and corneal staining with fluorescein and lissamine green dye) in patients with blepharospasm.
Dubravka Biuk, Maja Vinković, Suzana Matić, Mario Bradvica, Egon Biuk, Tvrtka Benašić   +5 more
doaj   +1 more source

Single-Cell Labeling Strategies to Dissect Neuronal Structures and Local Functions

Biology, 2023
The brain network consists of ten billion neurons and is the most complex structure in the universe. Understanding the structure of complex brain networks and neuronal functions is one of the main goals of modern neuroscience. Since the seminal invention
Keigo Kohara, Masayoshi Okada
doaj   +1 more source

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos [PDF]

Journal of Visualized Experiments, 2015
Methyl green has long been known as a histological stain with a specific affinity for DNA, although its fluorescent properties have remained unexplored until recently. In this article, we illustrate the method for preparing a methyl green aqueous stock solution, that when diluted can be used as a very convenient fluorescent nuclear label for fixed ...
Prieto Mena, Daniel, Aparicio Díaz, Hector Gonzalo, Machado, Matías, Zolessi, Flavio R.   +3 more
openaire   +3 more sources

Neutralization of the PTX3/CD44 axis suppresses TGF‐β1 signaling in kidney fibrosis

FEBS Letters, EarlyView.
This study investigates how the PTX3/CD44 axis promotes kidney fibrosis by activating TGF‐β1 signaling. We demonstrate that the neutralizing antibody WHC‐001 effectively inhibits this pathway, suppressing myofibroblast activation and partial epithelial–mesenchymal transition (EMT).
Chao‐Chun Cheng, Shen‐Shin Chang, Feng‐Wei Chen, Ying‐Chieh Su, Thanh Phuong Nguyen, Yu‐Wei Hsiao, Jhih‐Ying Chi, Hsin‐Yin Liang, Ju‐Ming Wang   +8 more
wiley   +1 more source

EdU and WGA labeling.

, 2013
Transversal section of a tentacle showing histological labeling of EdU+ nuclei (green) and mucocytes (arrow) stained with WGA (red). DAPI staining (blue) was used to visualize nuclei. E, endodermis; G, gastrodermis.
Ove Hoegh-Guldberg (150861), Anne-Catherine Herman (415960), Linda Tonk (415961), Stéphane Roberty (415959), Jean-Christophe Plumier (88146), David Fransolet (415958)   +5 more
core   +1 more source

Superresolution Imaging Through Single-Molecule Labeling

, 2023
Single-molecule localization microscopy (SMLM) is an advanced fluorescence microscopy technique that enables subdiffraction-resolution imaging of biological structures.
Hirushi Gunasekara Kalu Arachchige (17091895)
core   +1 more source

CHROMOENDOSCOPY USING TOLUIDINE BLUE PLUS LUGOL’S SOLUTION FOR EARLY DIAGNOSIS OF ESOPHAGEAL PREMALIGNANT LESIONS AND SUPERFICIAL NEOPLASMS IN HIGH-RISK PATIENTS

Arquivos de Gastroenterologia, 2019
BACKGROUND: Esophageal cancer is the eighth most common cancer. The prognosis is bleak in patients with advanced stages. Patients with early disease have a better prognosis than those with advanced stage.
Jean Félix PIÑERÚA-GONSÁLVEZ, Rosanna del Carmen ZAMBRANO-INFANTINO, Sylvia BENÍTEZ   +2 more
doaj   +1 more source