Expression of the CD6 T lymphocyte differentiation antigen in normal human brain [PDF]
, 1990 Antigens shared by the immune and central nervous systems (CNS) have been described repeatedly. The present study reports the expression of the CD6 lymphocyte differentiation antigen in normal human brain evidenced by immunohistochemistry and Northern ...
Mayer, Barbara +4 more
core +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
Surface Characterization of Biomaterials by Immunogold Staining - Quantitative Analysis [PDF]
, 1986 The labeling of target proteins by immunogold particles has been analyzed based on Einstein\u27s law of Brownian motion. The theory was confirmed from the experiments which employed antifibrinogen gold markers to label fibrinogen molecules adsorbed on ...
Simmons, Scott R. +2 more
core +1 more source
In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]
, 2010 We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Candrian, Christian +9 more
core +2 more sources
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Evaluation of different decalcifying agents on histochemical and immunohistochemical staining properties of canine osseous tissue [PDF]
Medical Laboratory JournalBackground: The purpose of tissue processing is to strengthen the tissue and place it in a suitable environment for slicing without causing damage.
Reyhaneh Shafieian +2 more
doaj
, 1993 Staining by uranyl acetate and lead citrate (UA-LC) of immunolabeled sections of unfixed glomerular basement membrane (GBM) digests embedded in low-acid glycol methacrylate (LA-GMA) is poor.
J W Stirling
core +1 more source
Automated segmentation of tissue images for computerized IHC analysis [PDF]
, 2010 This paper presents two automated methods for the segmentation ofimmunohistochemical tissue images that overcome the limitations of themanual approach aswell as of the existing computerized techniques.
Enrico Macii +6 more
core +1 more source
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
Sensitivity of Diagnostic Tests for Dry Eye in Patients with Blepharospasm
Acta Clinica Croatica, 2017 The aim of the study was to evaluate diagnostic tests for keratoconjunctivitis sicca (Schirmer test, tear break-up time (TBUT) test, and corneal staining with fluorescein and lissamine green dye) in patients with blepharospasm.
Dubravka Biuk +5 more
doaj +1 more source