Results 41 to 50 of about 339,106 (262)

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Evaluation of different decalcifying agents on histochemical and immunohistochemical staining properties of canine osseous tissue [PDF]

Medical Laboratory Journal
Background: The purpose of tissue processing is to strengthen the tissue and place it in a suitable environment for slicing without causing damage.
Reyhaneh Shafieian, Alireza Ebrahimzadeh-Bideskan, Esmail Ranjbar   +2 more
doaj  

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Sensitivity of Diagnostic Tests for Dry Eye in Patients with Blepharospasm

Acta Clinica Croatica, 2017
The aim of the study was to evaluate diagnostic tests for keratoconjunctivitis sicca (Schirmer test, tear break-up time (TBUT) test, and corneal staining with fluorescein and lissamine green dye) in patients with blepharospasm.
Dubravka Biuk, Maja Vinković, Suzana Matić, Mario Bradvica, Egon Biuk, Tvrtka Benašić   +5 more
doaj   +1 more source

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos [PDF]

Journal of Visualized Experiments, 2015
Methyl green has long been known as a histological stain with a specific affinity for DNA, although its fluorescent properties have remained unexplored until recently. In this article, we illustrate the method for preparing a methyl green aqueous stock solution, that when diluted can be used as a very convenient fluorescent nuclear label for fixed ...
Prieto Mena, Daniel, Aparicio Díaz, Hector Gonzalo, Machado, Matías, Zolessi, Flavio R.   +3 more
openaire   +3 more sources

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

CHROMOENDOSCOPY USING TOLUIDINE BLUE PLUS LUGOL’S SOLUTION FOR EARLY DIAGNOSIS OF ESOPHAGEAL PREMALIGNANT LESIONS AND SUPERFICIAL NEOPLASMS IN HIGH-RISK PATIENTS

Arquivos de Gastroenterologia, 2019
BACKGROUND: Esophageal cancer is the eighth most common cancer. The prognosis is bleak in patients with advanced stages. Patients with early disease have a better prognosis than those with advanced stage.
Jean Félix PIÑERÚA-GONSÁLVEZ, Rosanna del Carmen ZAMBRANO-INFANTINO, Sylvia BENÍTEZ   +2 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

STAINING TOXOPLASMA GONDII WITH FLUORESCEIN-LABELLED ANTIBODY [PDF]

The Journal of Experimental Medicine, 1957
Antitoxoplasma globulin was labelled with fluorescein and was used to stain Toxoplasma gondii organisms in smears of peritoneal exudate. The evidence indicates that this staining was due to an antigen-antibody reaction at the cellular level. Methods are presented for handling the organisms so that they can be stained over a period of several months ...
openaire   +2 more sources