SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Hepatic hydatid disease with spontaneous rupture into the ileum: a rare case report. [PDF]
Bennasser A +10 more
europepmc +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Using a neural network approach and starspots dependent models to predict effective temperatures and ages of young stars. [PDF]
Tarantino M +4 more
europepmc +1 more source
SKA tomography of Galactic star-forming regions and spiral arms [PDF]
Laurent Loinard +14 more
openalex +1 more source
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso +4 more
wiley +1 more source
Dynamic Die-Forging Scene Semantic Segmentation via Point Cloud-BEV Feature Fusion with Star Encoding. [PDF]
Feng X +7 more
europepmc +1 more source
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima +4 more
wiley +1 more source
The Connection Between Galaxy Environment and the Luminosity Function Slopes of Star-Forming Regions
D. Cook +5 more
openalex +1 more source
On the kinematics of massive star forming regions: the case of IRAS 17233–3606 [PDF]
S. Leurini +5 more
openalex +1 more source

