Results 51 to 60 of about 41,054 (268)

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

ALMA-IMF. XIV. Free–Free Templates Derived from H41α and Ionized Gas Content in 15 Massive Protoclusters

The Astrophysical Journal Supplement Series
We use the H41 α recombination line to create templates of the millimeter free–free emission in the ALMA-IMF continuum maps, which allows us to separate it from dust emission.
Roberto Galván-Madrid, Daniel J. Díaz-González, Frédérique Motte, Adam Ginsburg, Nichol Cunningham, Karl M. Menten, Mélanie Armante, Mélisse Bonfand, Jonathan Braine, Timea Csengeri, Pierre Dell’Ova, Fabien Louvet, Thomas Nony, Rudy Rivera-Soto, Patricio Sanhueza, Amelia M. Stutz, Friedrich Wyrowski, Rodrigo H. Álvarez-Gutiérrez, Tapas Baug, Sylvain Bontemps, Leonardo Bronfman, Manuel Fernández-López, Antoine Gusdorf, Atanu Koley, Hong-Li Liu, Javiera Salinas, Allison P. M. Towner, Anthony P. Whitworth   +27 more
doaj   +1 more source

Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation

FEBS Letters, EarlyView.
The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt, Marcus S. Münchhoff, Jacqueline Krohn, Philip M. Palarz, Manuel H. Taft, Johannes N. Greve, Nataliya Di Donato, Falk F. R. Buettner, Dietmar J. Manstein   +8 more
wiley   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Large-scale Mapping Observations of DCN and DCO+ toward Orion KL

The Astrophysical Journal
We present emission maps ( $1\buildrel{\,\prime}\over{.} 5\,\times \,1\buildrel{\,\prime}\over{.} 5$ scale, corresponding to 0.18 pc) of the DCN ( J = 2 − 1) and DCO ^+ ( J = 2 − 1) lines in the 2 mm band toward the Orion KL region obtained with the 2 mm
Kotomi Taniguchi, Prathap Rayalacheruvu, Teppei Yonetsu, Tatsuya Takekoshi, Bunyo Hatsukade, Kotaro Kohno, Tai Oshima, Yoichi Tamura, Yuki Yoshimura, Víctor Gómez-Rivera, Sergio Rojas-García, Arturo I. Gómez-Ruiz, David H. Hughes, F. Peter Schloerb, Liton Majumdar, Masao Saito, Iván Rodríguez-Montoya, David Sánchez-Arguelles, Ryohei Kawabe   +18 more
doaj   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Unlocking the potential of tumor‐derived DNA in urine for cancer detection: methodological challenges and opportunities

Molecular Oncology, EarlyView.
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever, Renske D. M. Steenbergen   +1 more
wiley   +1 more source

Surfaceome: a new era in the discovery of immune evasion mechanisms of circulating tumor cells

Molecular Oncology, EarlyView.
In the era of immunotherapies, many patients either do not respond or eventually develop resistance. We propose to pave the way for proteomic analysis of surface‐expressed proteins called surfaceome, of circulating tumor cells. This approach seeks to identify immune evasion mechanisms and discover potential therapeutic targets. Circulating tumor cells (
Doryan Masmoudi, Jérome Vialaret, Christophe Hirtz, Catherine Alix‐Panabières   +3 more
wiley   +1 more source