Results 111 to 120 of about 25,057 (234)

Using confocal microscopy and image processing to measure properties of sub‐resolution cell structures: Application to cardiac transverse tubules

Journal of Microscopy, EarlyView.
Abstract Confocal microscopes increase the resolution of images by rejecting unwanted data from the out of focus regions of the specimen. This incurs a penalty in the recorded signal to noise ratio that may be partially ameliorated by inputting additional information during image processing.
Mark B. Cannell, Cherrie H. T. Kong
wiley   +1 more source

Scanning gCW-STED nanoscopy images.

, 2013
Images of (A) ∼ 40-nm large fluorescent crimson beads, (b) ∼ 35-nm large FNDs, and (c) ATTO647N-immunostained microtubules of a fixed PtK2 cell. The insets (A,B) show a magnified view of the areas marked in the left images.
Stefan W. Hell (101918), Gael Moneron (101912), Haisen Ta (101910), Christian Eggeling (101915), Giuseppe Vicidomini (101908), Andreas Schönle (101909), Kyu Young Han (101911)   +6 more
core   +1 more source

Expansion Microscopy Reveals the Spatial Association of Therapeutic Antibody–Receptor Complexes With Cholesterol‐Rich Membrane Domains

Angewandte Chemie, Volume 138, Issue 42, 12 October 2026.
We report a super‐resolution strategy that maps cholesterol‐rich membrane domains in whole, intact cells with an effective spatial resolution of ∼40 nm by combining expansion microscopy with a perfringolysin O domain 4 probe. This approach enables direct visualization of the spatial association between CD20 and CD38 complexes and cholesterol‐rich ...
Gang Wen, Nicole Seifert, Patrick Eiring, Johanna Meyer, Hannes Neuweiler, Julia Weingart, Ignacio Vega‐Vásquez, Sören Doose, Philip Kollmannsberger, Markus Sauer   +9 more
wiley   +2 more sources

Multiplexed stimulated emission depletion nanoscopy (mSTED) for 5-color live-cell long-term imaging of organelle interactome

Opto-Electronic Advances
Stimulated emission depletion microscopy (STED) holds great potential in biological science applications, especially in studying nanoscale subcellular structures.
Yuran Huang, Zhimin Zhang, Wenli Tao, Yunfei Wei, Liang Xu, Wenwen Gong, Jiaqiang Zhou, Liangcai Cao, Yong Liu, Yubing Han, Cuifang Kuang, Xu Liu   +11 more
doaj   +1 more source

METODA STED CA INSTRUMENT DE ARMONIZARE A RELAȚIEI DINTRE PERFORMANȚA EDUCAȚIEI PROFESIONALE ȘI PIAȚA MUNCII ÎN REPUBLICA MOLDOVA – OPORTUNITĂȚI DE APLICARE ȘI LECȚII DE ÎNVĂȚAT

Studia Universitatis Moldaviae: Stiinte Umanistice, 2021
Obiectivul de bază al investigației a constat în elucidarea esenței și aspectelor aplicative ale metodei STED ca instru- ment de adaptare optimă a ofertei educaționale, în aspect cantitativ și calitativ, la necesitățile pieței muncii și, implicit, de ...
USM ADMIN
doaj  

The Origins of Ploem's Filter Cube: A Pandora's Box

Journal of Microscopy, EarlyView.
Abstract Ploem's filter cube, central to modern epifluorescence microscopy, integrates three matched optical components: an excitation filter, an emission filter, and a dichroic mirror. Together, these elements enable the separation of excitation and emission light paths, producing high‐contrast fluorescence images.
Jack Gordon, Kaleah Balcomb, Vinod Maseedupally, Maitreyee Roy   +3 more
wiley   +1 more source

Neurotrophic extracellular matrix proteins promote neuronal and iPSC astrocyte progenitor cell‐ and nano‐scale process extension for neural repair applications

Journal of Anatomy, Volume 246, Issue 4, Page 585-601, April 2025.
The extracellular matrix plays a critical role in modulating cell behaviour in the central nervous system influencing neural cell morphology and growth. However, a better understanding of the impact of individual matrix proteins on both neurons and astrocytes is critical for advancing the development of matrix‐based neural repair strategies.
Cian O'Connor, Rena E. Mullally, Sarah F. McComish, Julia O'Sullivan, Ian Woods, Ingmar Schoen, Massimiliano Garre, Maeve A. Caldwell, Adrian Dervan, Fergal J. O'Brien   +9 more
wiley   +1 more source

Localizing Axial Dense Emitters Based on Single-Helix Point Spread Function and Deep Learning

IEEE Photonics Journal
The point-by point 3D scanning strategy adopted in Stimulated Emission Depletion Microscopy (STED) is time-consuming. The 3D scanning can be replaced with a 2D scanning in the non-diffracting Bessel-Bessel STED (BB-STED).
Yihong Ji, Danni Chen, Hanzhe Wu, Gan Xiang, Heng Li, Bin Yu, Junle Qu   +6 more
doaj   +1 more source

Single‐Nucleus Transcriptome Analysis Provides New Insights Into B Chromosome Elimination in Sorghum

Plant Biotechnology Journal, EarlyView.
ABSTRACT B chromosomes are supernumerary entities found in many plant species, with some exhibiting tissue‐specific elimination. In Sorghum purpureosericeum, extensive B chromosome elimination occurs during embryogenesis affecting most of the embryonic organs, leaving the B chromosome maintained mainly in limited regions of meristems.
T. Bojdová, P. Abaffy, L. Valihrach, M. Karafiátová, J. Bartoš   +4 more
wiley   +1 more source

Analytical description of STED microscopy performance. [PDF]

, 2010
Stimulated emission depletion (STED) resolves fluorescent features that are closer than the far-field optical diffraction limit by applying a spatially modulated light field keeping all but one of these features dark consecutively.
Christian Eggeling, Eggeling, C., Eggeling, C, Hell, Stefan W., Hell, SW, Leutenegger, M, Stefan W. Hell, Hell, S. W., Hell, S., Leutenegger, M., Leutenegger, Marcel, Hell, Stefan, Marcel Leutenegger, Eggeling, Christian   +13 more
core   +1 more source