Results 121 to 130 of about 99,537 (336)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Sample Size Calculation for an Individual Stepped‐Wedge Randomized Trial

Biometrical Journal
ABSTRACTIn the individual stepped‐wedge randomized trial (ISW‐RT), subjects are allocated to sequences, each sequence being defined by a control period followed by an experimental period. The total follow‐up time is the same for all sequences, but the duration of the control and experimental periods varies among sequences. To our knowledge, there is no
Allemang-Trivalle, Aude, Maruani, Annabel, Giraudeau, Bruno   +2 more
openaire   +5 more sources

Stepped wedge trials with continuous recruitment require new ways of thinking [PDF]

Journal of Clinical Epidemiology, 2019
There is substantial variation in the design of stepped wedge trials. Many recruit participants continuously over time, although the methodological literature has tended not to differentiate closely between continuous recruitment and discrete sampling.
Hooper, R, Copas, A
openaire   +4 more sources

Pioglitazone plus (−)‐epigallocatechin gallate: a novel approach to enhance osteogenic performance in aged bone marrow mesenchymal stem cells

FEBS Open Bio, EarlyView.
Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen, Jia‐Ci Jhang, Chun‐Yi Peng, Quốc Cường Nguyễn, Feng‐Huei Lin, Hung‐Ming Wu, Shankung Lin   +6 more
wiley   +1 more source

Designing stepped wedge trials to evaluate physical activity interventions in schools: methodological considerations

International Journal of Behavioral Nutrition and Physical Activity
Background In a stepped wedge design, schools are randomised to a sequence of measurements, with each sequence transitioning to intervention status at a different time.
Ruth Salway, Danielle House, Simona Kent-Saisch, Robert Walker, Lydia Emm-Collison, Alice Porter, David R Lubans, Michael Beets, Frank de Vocht, Russell Jago   +9 more
doaj   +1 more source

Design of the HIV Prevention Trials Network (HPTN) Protocol 054: A cluster randomized crossover trial to evaluate combined access to Nevirapine in developing countries [PDF]

, 2003
HPTN054 is a cluster randomized trial designed to compare two approaches to providing single dose nevirapine to HIV-seropositive mothers and their infants to prevent mother-to-child transmission of HIV in resource limited settings.
Goldenberg, Robert L., Guay, Laura A., Hughes, Jim, Mmiro, Francis, Mwinga, Kasonde G., Stringer, Jeffrey S. A., Valentine, Megan, Wilfert, Catherine M.   +7 more
core   +1 more source

Promoting Recruitment using Information Management Efficiently (PRIME): study protocol for a stepped wedge cluster randomised controlled trial within the REstart or STop Antithrombotics Randomised Trial (RESTART) [PDF]

, 2017
BACKGROUND: Research into methods to boost recruitment has been identified as the highest priority for randomised controlled trial (RCT) methodological research in the United Kingdom.
Dennis, Martin, Maxwell, Amy, Parker, Richard, Rudd, Anthony, Salman, Rustam, Weir, Christopher   +5 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Enhancing Recruitment Using Teleconference and Commitment Contract (ERUTECC): study protocol for a randomised, stepped-wedge cluster trial within the EFFECTS trial

Trials, 2018
Background Many randomised controlled trials (RCTs) fail to meet their recruitment goals in time. Trialists are advised to include study recruitment strategies within their trials. EFFECTS is a Swedish, academic-led RCT of fluoxetine for stroke recovery.
Erik Lundström, Eva Isaksson, Per Wester, Ann-Charlotte Laska, Per Näsman   +4 more
doaj   +1 more source

Stepped wedge cluster randomised trials: a review of the statistical methodology used and available [PDF]

, 2016
Background: Previous reviews have focussed on the rationale for employing the stepped wedge design (SWD), the areas of research to which the design has been applied and the general characteristics of the design.
Barker, D., Campbell, M.J., D’Este, C., McElduff, P.   +3 more
core   +3 more sources