Results 71 to 80 of about 202,929 (259)

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

A systematic map of studies testing the relationship between temperature and animal reproduction

Ecological Solutions and Evidence
Exposure to extreme temperatures can negatively affect animal reproduction, by disrupting the ability of individuals to produce any offspring (fertility), or the number of offspring produced by fertile individuals (fecundity).
Liam R. Dougherty, Fay Frost, Maarit I. Maenpaa, Melissah Rowe, Benjamin J. Cole, Ramakrishnan Vasudeva, Patrice Pottier, Eva Schultner, Erin L. Macartney, Ina Lindenbaum, Jamie L. Smith, Pau Carazo, Marco Graziano, Hester Weaving, Berta Canal Domenech, David Berger, Abhishek Meena, Tom Rhys Bishop, Daniel W. A. Noble, Pedro Simões, Julian Baur, Merel C. Breedveld, Erik I. Svensson, Lesley T. Lancaster, Jacintha Ellers, Alessio N. De Nardo, Marta A. Santos, Steven A. Ramm, Szymon M. Drobniak, Matteo Redana, Cristina Tuni, Natalie Pilakouta, Z. Valentina Zizzari, Graziella Iossa, Stefan Lüpold, Mareike Koppik, Regan Early, Clelia Gasparini, Shinichi Nakagawa, Malgorzata Lagisz, Amanda Bretman, Claudia Fricke, Rhonda R. Snook, Tom A. R. Price   +43 more
doaj   +1 more source

ORGANIZATION AND ANALYSIS OF THE COMMISSION'S WORK ON THE SELECTION AND REFERRAL OF PATIENTS IN A MEDICAL INSTITUTION FOR TREATMENT USING ASSISTED REPRODUCTIVE TECHNOLOGIES ON THE TERRITORY OF THE CHELYABINSK REGION

Фармакоэкономика, 2015
For the purpose of improvement of quality of delivery of health care to women with infertility at Ministry of Health of Chelyabinsk region the Commission on selection and the direction of patients in the medical organizations for carrying out treatment ...
M. G. Moskvicheva, V. V. Saharova, Yu. A. Semenov, E. V. Orehova   +3 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Mars Viking Biology Instrument Sterility [PDF]

, 1973
A memorandum regarding biological contamination written by Richard Young, subject heading Viking Biology Instrument ...
Young, Richard
core   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

X-ray fluorescence characterization of some medicinal plants traditionally used as aphrodisiac in the treatment of male sterility in Kenge City, Democratic Republic of the Congo

Orapuh Journal
Introduction Male infertility refers to the inability of a man to impregnate a woman. It is a public health issue among men of reproductive age. Certain minerals, such as zinc, selenium, iron, and magnesium, are essential to produce healthy sperm and ...
Frederick Ndombe Mulwele, Koto-Te-Nyiwa Ngbolua, B Yang Da Musa Masens, Théophile Fundu Mbemba, Pius Tshimankinda Mpiana   +4 more
doaj   +1 more source

Microbial Quality of Dehydrated Media Used in the Sterility Testing of Immunobiological Medicinal Products

Биопрепараты: Профилактика, диагностика, лечение, 2018
Medicinal products fail sterility testing if visual observation shows the growth of microorganisms that manifests itself as turbidity, sedimentation, flocculation and other changes in the growth medium.
S. M. Sukhanova, N. E. Zakharova
doaj   +1 more source