Results 41 to 50 of about 235,966 (268)
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
ES-TRNG: A High-throughput, Low-area True Random Number Generator based on Edge Sampling
Transactions on Cryptographic Hardware and Embedded Systems, 2018 In this paper we present a novel true random number generator based on high-precision edge sampling. We use two novel techniques to increase the throughput and reduce the area of the proposed randomness source: variable-precision phase encoding and ...
Bohan Yang +4 more
doaj +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Efficiency evaluation in public road transport: a stochastic frontier analysis
Transport, 2015 This paper measures the technical efficiency of 54 public road transport operators and investigates the degree to which various factors influence efficiency levels in these firms. The study makes an attempt to provide an overview of the general status of
Sami Jarboui +2 more
doaj +1 more source
Stochastic Model Checking [PDF]
, 2007 This tutorial presents an overview of model checking for both discrete and continuous-time Markov chains (DTMCs and CTMCs). Model checking algorithms are given for verifying DTMCs and CTMCs against specifications written in probabilistic extensions of temporal logic, including quantitative properties with rewards.
Marta Z. Kwiatkowska +2 more
openaire +1 more source
Molecular Oncology, EarlyView.Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller +10 more
wiley +1 more source
Postsynaptic Stability and Variability Described by a Stochastic Model of Endosomal Trafficking
Frontiers in Cellular Neuroscience, 2019 Neurons undergo dynamic processes of constitutive AMPA-type glutamate receptor (AMPAR) trafficking, such as the insertion and internalization of AMPARs by exocytosis and endocytosis, while stably maintaining synaptic efficacy.
Taegon Kim +2 more
doaj +1 more source
Molecular Oncology, EarlyView.Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley +1 more source
Multi-Objective Closed-loop Supply Chain Considering Vehicles and Solving by New Approaches in Metaheuristics [PDF]
مجله مدل سازی در مهندسی, 2019 Recently, concerns have been raised about the environmental and social impacts of commercial activities. Also, most papers on the design of the supply chain network, focus on economic performance.
Anita Abdi, Mostafa Hajiaghaei-Keshteli
doaj +1 more source