Results 81 to 90 of about 74,695 (306)
The evolution of alternative migratory strategies can lead to the establishment of ecologically and genetically distinct morphs; however, the forces that maintain these differences are often unclear.
C. S. T. MacPhail +4 more
doaj +1 more source
The Economics of Rotational Grazing in the Gulf Coast Region: Costs, Returns, and Labor Considerations, Phase II [PDF]
Profitability and labor associated with rotational grazing at three stocking rates and continuous grazing at a medium stocking rate are compared. On a per-acre basis, profits are lowest for low stocking rate rotational grazing.
Blouin, David +6 more
core
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
STOCKING OF PEN‐REARED “WILD” TURKEYS BY THE PUBLIC: A NATIONWIDE SURVEY OF STATE WILDLIFE AGENCIES
: A questionnaire to determine the extent of the problem of releases of pen‐reared “wild” turkeys (Meleagris gallopavo) by the public was mailed to state wild turkey project leaders in 49 states in 1992‐93. Responses were received from all 49.
William G. Minser +2 more
doaj +1 more source
This study aimed to evaluate the productive parameters of pearl millet (Pennisetum glaucum (L.) R. BR) and Sudan grass (Sorghum bicolor cv. Sudanense) pastures, under continuous grazing by cows destined to slaughter.
Rangel Fernandes Pacheco +5 more
doaj +1 more source
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source

