Results 61 to 70 of about 121,925 (266)
Molecular Oncology, EarlyView.Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller +10 more
wiley +1 more source
Remote Sensing, 2018 We present a new method for real-time runway detection embedded in synthetic vision and an ROI (Region of Interest) based level set method. A virtual runway from synthetic vision provides a rough region of an infrared runway.
Changjiang Liu, Irene Cheng, Anup Basu
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Discrete Dynamics in Nature and Society, 2012 This paper presents a 2D convergence density criterion for minimizing the total junction delay at isolated junctions in the lane-based optimization framework.
C. K. Wong, Y. Y. Lee
doaj +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
Bayesian sequential estimation of the proportion of damage in maize seeds
Scientia AgricolaSampling is an essential step in estimating a parameter: thus, cost and time associated to this step should be minimized. Sequential sampling is characterized by using samples of variable sizes given as a function of observations, and sequential sampling
Isabela da Silva Lima +2 more
doaj +1 more source
Identifying Key Success Factors in Stopping Flaky Tests in Automated REST Service Testing
Journal of Computer Science and Technology, 2018 A flaky test is a test which could fail or pass for the same version of a certain software code. In continuous software development environments, flaky tests represent a problem.
Maximiliano A. Mascheroni +1 more
doaj +1 more source