Results 91 to 100 of about 76,097 (293)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Agreement Between the Stages Cycling and SRM Powermeter Systems during Field-Based Off-Road Climbing. [PDF]

open access: yes, 2015
The aim of this study was to determine the agreement between two portable cycling powermeters for use doing field based mountain biking. A single participant performed 15 timed ascents of an off-road climbs. The participants bicycle was instrumented with
Atkins, Stephen   +3 more
core  

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

open access: yesFEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

Stress-strain synchronization for high strain rate tests on brittle composites [PDF]

open access: yes, 2018
Nowadays, many researchers develop rate-dependent composite material models for application in dynamic simulations. Ideally, full stress-strain curves at a wide range of strain rates are available for identification of the different parameters of these ...
Garoz Gómez, David   +6 more
core   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

open access: yesFEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima   +4 more
wiley   +1 more source

Structural performance of steel-concrete sandwich beams with carbon nanofiber reinforcement [PDF]

open access: yes, 2019
Cementitious materials such as concrete are typically characterised as quasi-brittle with low tensile strength and low strain capacity, which hence affect the long-term durability of the structure.
Ashraf S. Ayoub   +19 more
core   +1 more source

Antibiofilm activity of a chionodracine‐derived peptide by NMR‐based metabolomics of cell‐free supernatant of Acinetobacter baumannii clinical strains

open access: yesFEBS Open Bio, EarlyView.
KHS‐Cnd peptide is able to impair biofilm formation and disaggregate mature biofilms in Acinetobacter baumannii clinical isolates. Differences in extracellular metabolites reflect changes in biofilm metabolism due to KHS‐Cnd treatment. Among the differentially represented extracellular metabolites upon KHS‐Cnd treatment, the significantly altered ...
Fernando Porcelli   +9 more
wiley   +1 more source

Silicon whisker crystal-based strain gauges for low-temperature applications.

open access: yesТехнологія та конструювання в електронній апаратурі, 2008
The strain characteristics of p-type silicon whisker crystals have been investigated in the temperature range of 4.2 – 300 K. On their basis, strain gauges with an anomalously high gauge factor at 4.2 K have been developed for measuring deformation in ...
A. A. Druzhinin   +3 more
doaj  

A new device for stress monitoring in continuously welded rails using bi-directional strain method

open access: yesJournal of Modern Transportation, 2018
The technology of continuously welded rails (CWRs) is important in modern railway track structures. To measure rail stress, resistance strain gauges are preferred due to their good stability, sensitivity, and resistance to external interference. Based on
Ganzhong Liu   +5 more
doaj   +1 more source

Determination of the technical constants of laminates in oblique directions [PDF]

open access: yes
An off-axis tensile test theory based on Hooke's Law is applied to glass fiber reinforced laminates. A corrective parameter dependent on the characteristics of the strain gauge used is introduced by testing machines set up for isotropic materials ...
Vidouse, F.
core   +1 more source

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