Results 71 to 80 of about 388,695 (254)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Lower-extremity muscle strength is associated with prognosis in patients with advanced or recurrent lung cancer: a retrospective, observational study

BMC Cancer
Background We aimed to examine associations between various sarcopenia indices—including skeletal muscle index (SMI), handgrip strength, lower-extremity muscle strength, a combined measure of handgrip and lower-extremity muscle strength, sarcopenia ...
Takuya Fukushima, Utae Katsushima, Naoya Ogushi, Kimitaka Hase, Jiro Nakano   +4 more
doaj   +1 more source

Characteristics of palm oil fuel ash concrete admixed with precipitated silica and silica fume

Cleaner Engineering and Technology
This study investigates the characteristics of blended Palm Oil Fuel Ash (POFA) concrete admixed with silica fume (SF) and precipitated silica (SP) as a cement replacement material for Portland Composite Cement (PCC).
Monita Olivia, M Aldi Maulidi, Fadhlurrahman, Gunawan Wibisono   +3 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Factors of the effectiveness of competitive activity of arm wrestlers aged 14-15 years at the stage of preliminary basic training

Слобожанський науково-спортивний вісник
Background and Study Aim. Considering that there are no studies aimed at identifying the components of physical and functional fitness in arm wrestling at the stage of preliminary basic training, and it is not advisable to transfer training of adult ...
Dmytro Bezkorovainyi, Artur Oderov, Kravchenko Anton, Leonid Plotnytskyi, Liudovyk Tetiana, Volodymyr Ozharevskyi   +5 more
doaj   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Correlation between Handgrip Strength, Mobilization Function, Physical Activity Level, and Muscle Mass in Community-Dwelling Elderly in Bandung, West Java Province, Indonesia

International Journal of Integrated Health Sciences, 2018
Objective: To investigate correlation between handgrip strength, mobilization function, and physical activity level (PAL), and muscle mass in community-dwelling Indonesian elderly.
Sari Sekarsari, Vitriana, Irma Ruslina Defi   +2 more
doaj   +1 more source