Results 81 to 90 of about 1,763,815 (268)
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Optimization of Composite Material Repair Patch Shape Based on Strength Analysis
Applied SciencesWith the increasing use of composite materials in aircraft structures and the extension of their service life, the selection of repair patch shapes for composite material damage repair has become a significant engineering concern.
Ruishan Xing +3 more
doaj +1 more source
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Material's Strength Analysis of the Coupling Node of Axle of the Truck Trailer. [PDF]
Materials (Basel), 2023 Decker Ž +7 more
europepmc +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Strength analysis and structure optimization of locomotive frame based on TB/T 3549.1—2019
机车电传动This paper compared and analyzed the differences in the lateral load of the frame and the inertia load of the suspension components between TB/T 3549.1—2019 and the standards UIC 615-4:2003 and EN 13749:2011.
DONG Shengmin, ZHANG Guoxin
doaj
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
Design of Large Torque Combined PDM for Pipeline Laying Trenchless Technology
Shiyou jixie, 2018 At present, PDM is mainly used to provide torque for the reaming bit in the trenchless technique for pipeline laying. However, owing to the limited torque provided by the existing PDM, multiple reaming operations are needed to achieve the requirements ...
Zhang Jie, Liang Zheng, Han Chuanjun
doaj