Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Influence of Stress Intensity Factor on Rail Fatigue Crack Propagation by Finite Element Method. [PDF]
Materials (Basel), 2021 Gao R, Liu M, Wang B, Wang Y, Shao W.
europepmc +1 more source
Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells
FEBS Open Bio, EarlyView.To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai +8 more
wiley +1 more source
Notch Effects on the Stress Intensity Factor and on the Fatigue Crack Path for Eccentric Circular Internal Cracks in Elliptically Notched Round Bars under Tensile Loading. [PDF]
Materials (Basel), 2022 Toribio J +3 more
europepmc +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Stress Intensity Factors for Embedded, Surface, and Corner Cracks in Finite-Thickness Plates Subjected to Tensile Loading. [PDF]
Materials (Basel), 2021 Toribio J +3 more
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
工程科学与技术, 2009 :An element initial stress method to calculate stress intensity factor of three-dimensional mixed-mode crack was introduced, which adopted the degradation of the 1 / 4 node singular element to get the stress intensity factor from calculating the stress ...
Fan Hong, 张盛, 王启智
doaj
Residual Notch Stress Intensity Factors in Welded Joints Evaluated by 3D Numerical Simulations of Arc Welding Processes. [PDF]
Materials (Basel), 2021 Campagnolo A +3 more
europepmc +1 more source
FEBS Open Bio, EarlyView.TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source