Results 41 to 50 of about 4,353 (213)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Experimental study of unloading impact on deformation behavior and strength of mudstone in sampling state

Shuili Shuiyun Gongcheng Xuebao, 2017
The strength and deformation characteristics of mudstone are important reference indexes in the engineering design and construction management. In order to analyse the effect of water-rock interaction and unloading on the strength properties and ...
MA Furong, ZHANG Xingui, YI Nianping
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

A Simple Stress-Strain Relation Based on Stress-Path Behavior in Strain-Path Controlled Triaxial Tests

Soils and Foundations, 2003
ABSTRACT A new devise is developed for conducting a strain-path controlled test with a conventional triaxial test apparatus. By controlling lateral stress during axial strain controlled test, this devise can keep the principal strain increment ratio, Re , defined as a ratio between axial and lateral strain increments, constant. Toyoura sand specimens
K. Tokimatsu, Y. Asaka, K. Iwasaki, Y. Shamoto   +3 more
openaire   +2 more sources

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

Direct shear tests with evaluation of variable shearing area / Tiesioginio kirpimo bandymai įvertinant kintamą kerpamąjį plotą

Mokslas: Lietuvos Ateitis, 2014
Investigations of soil shear strength properties for Baltic Sea shore sand along Klaipėda city are presented. Investigated sand angle of internal friction (φ) and cohesion (c) is determined via two different direct shear tests procedures. First procedure
Šarūnas Skuodis, Tadas Tamošiūnas
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

Evolution Laws of Breakage for Gypsum Granular Materials

工程科学与技术, 2012
:To study the fractal evolution laws of particle breakage for gypsum granular materials under loading, three kinds of stress path tests were conducted with the triaxial apparatus.
Zhang Jianwei, Wang Chen, Zheng Qiwen
doaj