Results 221 to 230 of about 4,882,816 (314)

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

ClBCM: an EMS mutated gene regulating chlorophyll synthesis and adaptation to diverse stresses in watermelon. [PDF]

Mol Hortic
Fang T, Zhuang Z, Zhao M, Chu X, Tan J, Xu X, Cheng R, Zhang Y, Wang X, Xu Y, Zhang X, Deng Y.   +11 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Genome-wide identification, expression pattern and subcellular localization analysis of the WRKY gene family under abiotic stresses in Litchi chinensis. [PDF]

BMC Plant Biol
Yang J, Chen R, Liu W, Fan C.
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Reversibly-sealable microfluidic platform for multi-molecule gradient delivery to large adherent cell cultures. [PDF]

Biomed Microdevices
Radzio J, Suprewicz Ł, Kuang D, Karpowicz A, Janmey PA, Sul JY, Issadore DA, Eberwine JH, Arratia PE.   +8 more
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

ASME-Based Structural Assessment of Head-Shell Junctions in Pressurized Railway Tank Wagons. [PDF]

Materials (Basel)
Ilincă CN, Doukeh R, Ramadan IN, Neacsa A, Diniță A, Laudacescu EV, Petrescu MG, Ilie B, Sîrbu AC.   +8 more
europepmc   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

Foot progression angle modifications alter hip joint contact forces and femoral growth plate mechanics in children. [PDF]

Front Bioeng Biotechnol
Koller W, Wallnöfer E, Rathmair L, Egner C, Kitir K, Kainz H.   +5 more
europepmc   +1 more source