Results 61 to 70 of about 95,473 (252)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Paradoxical G-quadruplex distribution in coronavirus genomes reveals functional constraints and antiviral therapeutic opportunities

Virus Research
We computationally characterized G-quadruplex (G4) distributions across 31 coronavirus genomes to identify conserved structural features as potential antiviral therapeutic targets.
Masato Tanigawa, Takafumi Iwaki
doaj   +1 more source

An Optimally Stable and Accurate Second‐Order SSP Runge‐Kutta IMEX Scheme for Atmospheric Applications

Journal of Advances in Modeling Earth Systems, 2018
The objective of this paper is to develop an optimized implicit‐explicit (IMEX) Runge‐Kutta scheme for atmospheric applications focusing on stability and accuracy.
Arman Rokhzadi, Abdolmajid Mohammadian, Martin Charron   +2 more
doaj   +1 more source

Cubic trigonometric B-spline differential quadrature method for numerical treatment of Fisher’s reaction-diffusion equations

Alexandria Engineering Journal, 2018
This paper concerns through the numerical treatment of Fisher’s reaction-diffusion equation by using a hybrid numerical method. In this method, the combination of cubic trigonometric B-spline (CTB) base functions and differential quadrature method is ...
Mohammad Tamsir, Neeraj Dhiman, Vineet K. Srivastava   +2 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Enhanced perpendicular coercivity of ultrathin perpendicularly magnetized Tb–Fe–Co films on silicon substrates using a thin Pt underlayer

Journal of Science: Advanced Materials and Devices, 2016
We have studied the effect of an additional thin Pt underlayer on the magnetic properties of ultrathin perpendicularly magnetized TbFeCo films grown on silicon substrates.
Bang Do, Hiroyuki Awano
doaj   +1 more source

Strong Stability Preservation for Stochastic Partial Differential Equations

CoRR
This paper extends deterministic notions of Strong Stability Preservation (SSP) to the stochastic setting, enabling nonlinearly stable numerical solutions to stochastic differential equations (SDEs) and stochastic partial differential equations (SPDEs) with pathwise solutions that remain unconditionally bounded.
openaire   +2 more sources

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

A new four-step iteration scheme for generalized α-nonexpansive multivalued mappings in Banach spaces

Journal of Inequalities and Applications
In this paper, we introduce a new four-step iterative process for approximating fixed points of generalized α-nonexpansive multivalued mappings in uniformly convex Banach spaces.
Makbule Kaplan Özekes
doaj   +1 more source