Results 141 to 150 of about 425,057 (313)

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Mechanical fracture energy and structural-mechanical properties of meat snacks with beekeeping additives

open access: yesInformatyka, Automatyka, Pomiary w Gospodarce i Ochronie Środowiska
The choice of an effective technological scheme of food production is mainly determined by the need to meet consumer needs, healthy eating requirements, compliance with environmental trends, energy efficiency of the main processes of the technology used.
Artem Antoniv   +3 more
doaj   +1 more source

Determination of wood strength properties through standard test procedures

open access: yes, 2012
In this study a review of existing recognised standards for wood mechanical testing was conducted. This review considers tensile, compressive, bending and shear test methodologies from a range of sources.
Perera, Noel   +2 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Single Polymer Composites Based on Polypropylene: Processing and Properties

open access: yes, 2004
PhDIsotropic polymers lack sufficient strength and stiffness for many engineering applications. In order to improve these properties polymers can be filled with structural reinforcements such as glass or natural fibres.
Alcock, Benjamin
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

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