Results 131 to 140 of about 1,236,466 (297)

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Evaluation of Structural Stability, Mechanical Properties, and Corrosion Resistance of Magnesia Partially Stabilized Zirconia (Mg-PSZ). [PDF]

Molecules, 2023
Yusuf D, Maryani E, Mardhian DF, Noviyanti AR.   +3 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Self-assembling multiblock amphiphiles: Molecular design, supramolecular structure, and mechanical properties

, 2015
We perform off-lattice, canonical ensemble molecular dynamics simulations of the self-assembly of long segmented copolymers consisting of alternating, tunably attractive and hydrophobic {\em binder} domains, connected by hydrophilic {\em linker} chains ...
Mortazavi, Hamed, Storm, Cornelis
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

The Injection Molding of Biodegradable Polydioxanone-A Study of the Dependence of the Structural and Mechanical Properties on Thermal Processing Conditions. [PDF]

Polymers (Basel), 2022
Erben J, Blatonova K, Kalous T, Capek L, Behalek L, Boruvka M, Chvojka J.   +6 more
europepmc   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Mechanical fracture energy and structural-mechanical properties of meat snacks with beekeeping additives

Informatyka, Automatyka, Pomiary w Gospodarce i Ochronie Środowiska
The choice of an effective technological scheme of food production is mainly determined by the need to meet consumer needs, healthy eating requirements, compliance with environmental trends, energy efficiency of the main processes of the technology used.
Artem Antoniv, Igor Palamarchuk, Leonora Adamchuk, Marija Zheplinska   +3 more
doaj   +1 more source

Knockdown of biglycan reveals an important role in maintenance of structural and mechanical properties during tendon aging. [PDF]

J Orthop Res, 2023
Darrieutort-Laffite C, Beach ZM, Weiss SN, Eekhoff JD, Soslowsky LJ.   +4 more
europepmc   +1 more source