Results 91 to 100 of about 650,868 (292)

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

Polymorphism in magic-sized Au144(SR)60 clusters

open access: yesNature Communications, 2016
Gold nanoclusters are important nanomaterials but their structural assignment can be challenging if single crystals can’t be grown. Here, the authors use pair distribution function analysis of X-ray powder diffraction data for Au144(SR)60nanoclusters ...
Kirsten M.Ø. Jensen   +6 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Simultaneous structural and control optimization via linear quadratic regulator eigenstructure assignment [PDF]

open access: yes
A method for simultaneous structural and control design of large flexible space structures (LFSS) to reduce vibration generated by disturbances is presented.
Becus, G. A.   +3 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Integrating computer-aided modeling and micro-simulation in multi-criteria evaluation of service infrastructure assignment approaches

open access: yesJournal of Industrial Engineering and Management, 2013
Purpose: This paper proposes an integrated computer-supported multi-staged approach to the flexible design and multicriteria evaluation of service infrastructure assignment processes/ algorithms. Design/methodology/approach: It involves particularizing a
Alfonso Duran   +2 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

Automated Projection Spectroscopy (APSY) for the Assignment of NMR Resonances of Biological Macromolecules

open access: yesCHIMIA, 2012
High resolution nuclear magnetic resonance (NMR) spectroscopy in solution is an established technique in structural biology. Detailed functional and structural studies of biological macromolecules by NMR require the assignment of the chemical ...
Barbara Krähenbühl, Gerhard Wider
doaj   +1 more source

Inferring Interpersonal Relations in Narrative Summaries

open access: yes, 2015
Characterizing relationships between people is fundamental for the understanding of narratives. In this work, we address the problem of inferring the polarity of relationships between people in narrative summaries.
Chaturvedi, Snigdha   +2 more
core   +1 more source

Brainstem and Cerebellar Volume Loss and Associated Clinical Features in Progressive Supranuclear Palsy

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Introduction Progressive Supranuclear Palsy (PSP) is a neurodegenerative ‘tauopathy’ with predominating pathology in the basal ganglia and midbrain. Caudal tau spread frequently implicates the cerebellum; however, the pattern of atrophy remains equivocal.
Chloe Spiegel   +8 more
wiley   +1 more source

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