Results 111 to 120 of about 107,582 (301)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Ring-opening polymerization of ethylene carbonate: comprehensive structural elucidation by 1D & 2D-NMR techniques, and selectivity analysis

, 2017
Meticulous structural elucidation and selectivity analysis of poly(ethylene ether carbonate) by multi-dimensional NMR.
Muhammad Imran Malik, Rubina Abdul-Karim, Abdul Hameed   +2 more
core   +1 more source

Structural elucidation of low abundant metabolites in complex sample matrices

, 2013
Identification of metabolites is a major challenge in biological studies and relies in principle on mass spectrometry (MS) and nuclear magnetic resonance (NMR) methods.
Vervoort, J., Ridder, L.O., Vos, R.C.H., de, Hooft, J.J.J., van der, Bino, R.J.   +4 more
core   +2 more sources

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Diverse polyketides and alkaloids from Penicillium sp. KHMM: structural elucidation, biological and molecular docking studies.

, 2019
Hamed A, Ismail M, El-Metwally MM, et al. Diverse polyketides and alkaloids from Penicillium sp. KHMM: structural elucidation, biological and molecular docking studies. Zeitschrift für Naturforschung C.
Frese, Marcel ; https://orcid.org/, Ismail, Mohamed, El-Metwally, Mohammad M, Ibrahim, Tarek M A, El-Haddad, Atef F, Shaaban, Mohamed, Hamed, Abdelaaty, Sewald, Norbert ; https://orcid.org/   +7 more
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Tannin structural elucidation and quantitative31P NMR analysis. 1. Model compounds

, 2013
Tannins and flavonoids are secondary metabolites of plants that display a wide array of biological activities. This peculiarity is related to the inhibition of extracellular enzymes that occurs through the complexation of peptides by tannins.
Melone F, Saladino R. Lange H, Crestini C, Melone, F, Crestini, C, Saladino R., Lange H   +5 more
core   +1 more source

Chemical diversity from the Tibetan Plateau fungi Penicillium kongii and P. brasilianum

Mycology, 2018
Two new secondary metabolites, kongiilines A and B (1, 7), and two asperphenamate derivatives, asperphenamates B and C (5–6), together with 16 known compounds (2–4, 8–20), were isolated from Tibetan Plateau fungi Penicillium kongii and Penicillium ...
Zhi-Guo Liu, Li Bao, Hong-Wei Liu, Jin-Wei Ren, Wen-Zhao Wang, Long Wang, Wei Li, Wen-Bing Yin   +7 more
doaj   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Structural elucidation by electrospray mass spectrometry: an approach to the in vitro metabolism of the macrolide immunosuppressant SDZ RAD

, 1998
SDZ RAD [40-O-(2-hydroxyethyl)rapamycin] is a macrolide immunosuppressant that is currently under clinical investigation after organ transplantation. The elucidation of its metabolic pathway is essential to improve the understanding of its therapeutic ...
Kirchner, Gabriele I, Karl-Friedrich Sewing, Gabriele I. Kirchner, Christian Vidal, Vidal, Christian, Sewing, Karl-Friedrich   +5 more
core   +1 more source