Results 41 to 50 of about 1,624,721 (312)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Second Generation of Active Structural Control in Civil Engineering

Computer-Aided Civil and Infrastructure Engineering, 1996
: Research and development in active control of civil engineering structures in the United States has approximately a 20-year history. Under the leadership of the U.S. National Science Foundation in forming the U.S. Panel on Structural Control Research and in launching a program initiative in the area of structural control, there has been a surge of ...
G.W. Housner, T.T. Soong, S.F. Masri
openaire   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

A review of progressive collapse research and regulations [PDF]

, 2014
History has demonstrated that buildings designed to conventional design codes can lack the robustness necessary to withstand localised damage, partial or even complete collapse. This variable performance has led governmental organisations to seek ways of
Byfield, M.P., Morison, C., Mudalige, Wjesundara, Stoddart, E.   +3 more
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Analysis of a selected node of a truss made of cold-rolled sections based on the finite element method [PDF]

, 2018
The study presents a comparative analysis of displacements and reduced stresses that occur in the selected node of a truss made of cold-rolled sections. Computations were based on the finite element method using the Autodesk ROBOT Structural Analysis
Kalinowski, Jaroslaw, Kosin, Mariusz, Major, Izabela, Major, Maciej   +3 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Heat treatment design guided by semi-supervised learning for strength-ductility optimization in an Al-Cu-Li alloy

Materials & Design
This study presents a semi-supervised learning-guided strategy for designing a dual-stage aging schedule for an Al-Cu-Li alloy, enabling decoupled control of T1 and θ′ precipitates. By integrating active learning and self-training, the approach optimizes
Lyu Jing, Li Yanan, Li Xiwu, Xiao Wei, Chen Shuhai, Wen Kai, Liu Qilong, Yan Hongwei, Yu Mingyang, Li Zhihui, Zhang Yongan, Xiong Baiqing   +11 more
doaj   +1 more source

New or existing, does it matter?

Acta Polytechnica CTU Proceedings, 2022
Both when designing new and when evaluating existing structures, methods of analysis must be based on established engineering theory and practice. Also, the general principles of structural reliability regarding the treatment of uncertainties when ...
Peter Tanner, Ramon Hingorani, Carlos Lara   +2 more
doaj   +1 more source